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In this study, we demonstrate that Arabidopsis <jats:italic>CLE14<\/jats:italic> acts as a critical regulator of root hair elongation. Compared to the wild\u2010type (WT) plant, CRISPR\/Cas9\u2010generated <jats:italic>cle14\u2010cr<\/jats:italic> mutants exhibit reduced root hair length, whereas overexpression of <jats:italic>CLE14<\/jats:italic> promotes root hair elongation. In consistence, the synthetic CLE14 peptide also facilitates root hair elongation. However, CLE14 peptide\u2010induced root hair elongation is significantly compromised in <jats:italic>clv2<\/jats:italic> and <jats:italic>crn<\/jats:italic> mutants, suggesting the requirement of CLV2 and CRN proteins in CLE14\u2010mediated root hair growth. Genetic and pharmacological evidence further indicates that ethylene acts downstream of CLE14\u2010CLV2\/CRN module to regulate root hair elongation. Additionally, CLE14 peptide upregulates the expression level of <jats:italic>RHD6<\/jats:italic> and <jats:italic>RSL4<\/jats:italic> via EIN3 and EIL1, the key transcription factors in ethylene signalling. Subsequently, <jats:italic>RSL4<\/jats:italic> acts downstream of <jats:italic>RHD6<\/jats:italic> to trigger hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) and nitric oxide (NO) accumulation by upregulating the expression of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and NO biosynthesis\u2010related genes, ultimately driving root hair elongation. Collectively, our findings elucidate the signalling components underlying CLE14\u2010mediated root hair elongation.<\/jats:p>","DOI":"10.1111\/pce.15584","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T01:04:47Z","timestamp":1746666287000},"page":"6251-6268","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A CLE14 Signalling Cascade Promotes Arabidopsis Root Hair Elongation"],"prefix":"10.1111","volume":"48","author":[{"given":"Yong","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Bioscience and Bioengineering Jiangxi Agricultural University Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youxin","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory for Postharvest Storage and Preservation of Fruits &amp; Vegetables College of Agronomy, Jiangxi Agricultural University Nanchang 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