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Compared to the wild\u2010type and <jats:italic>FpCBS<\/jats:italic> complemented strains, the <jats:italic>FpCBS<\/jats:italic> deletion strain had a similar hyphal morphology and growth rate but reduced conidiation. In culture, the <jats:italic>FpCBS<\/jats:italic> deletion strain showed broad\u2010scale changes with a decrease in H<jats:sub>2<\/jats:sub>S levels, changes in sulphur\u2010containing amino acids and 1207 differentially expressed proteins. The deletion strain also showed reduced deoxynivalenol (DON) production, cellophane penetration ability, antioxidant capacity, as well as increased hyphal iron levels, sensitivity to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and lipid peroxidation. During infection, the \u0394<jats:italic>Fpcbs<\/jats:italic> strain showed diminished virulence to wheat with fewer penetration structures, and there was a stronger host defence response with wheat epidermal cells having thickened walls and greater reactive oxygen species accumulation. Transcriptomic analysis of infected leaves showed 1954 differentially expressed genes in the <jats:italic>FpCBS<\/jats:italic> deletion strain compared to the wild\u2010type strain. This included genes for sulphur metabolism, antioxidative defence, plant cell wall\u2010degrading enzymes and trichothecene production. <jats:italic>FpCBS<\/jats:italic> not only plays a pivotal role in sulphur metabolism and H<jats:sub>2<\/jats:sub>S production, but it is also important for a broad range of fungal functions, including iron homoeostasis, mycotoxin production and development (conidiation and penetration structures). These factors may all contribute to varying extents to the significant decrease in virulence of the <jats:italic>FpCBS<\/jats:italic> deletion strain. The findings have shown that <jats:italic>FpCBS<\/jats:italic> is important for much more than sulphur metabolism and H<jats:sub>2<\/jats:sub>S synthesis. However, its role in virulence highlights its potential as a novel target for developing strategies to control wheat crown rot.<\/jats:p>","DOI":"10.1111\/pce.15622","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T04:15:01Z","timestamp":1747973701000},"page":"6587-6604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>FpCBS<\/i> Affects a Diverse Range of Functions of <i>Fusarium pseudograminearum<\/i> Impacting Its Virulence to Wheat"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3812-1855","authenticated-orcid":false,"given":"Jie","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Biopesticide Engineering Research Center, Henan International Joint Laboratory of Crop Protection Zhengzhou P. R. 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