{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:17:18Z","timestamp":1787015838492,"version":"build-2736575974"},"reference-count":66,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The transport of CO\n                    <jats:sub>2<\/jats:sub>\n                    across biomembranes in plant cells is essential for efficient photosynthesis. Some aquaporins capable of CO\n                    <jats:sub>2<\/jats:sub>\n                    transport, referred to as \u2018COOporins\u2019, are postulated to play a crucial role in leaf CO\n                    <jats:sub>2<\/jats:sub>\n                    diffusion. However, the structural basis of CO\n                    <jats:sub>2<\/jats:sub>\n                    permeation through aquaporins remains largely unknown. Here, we show that amino acids in loop C are critical for the CO\n                    <jats:sub>2<\/jats:sub>\n                    permeability of\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    PIP2 aquaporins. We found that swapping tyrosine and serine in loop C to histidine and phenylalanine, which differ between AtPIP2;1 and AtPIP2;3, altered CO\n                    <jats:sub>2<\/jats:sub>\n                    permeability when examined in the\n                    <jats:italic>Xenopus laevis<\/jats:italic>\n                    oocyte heterologous expression system. AlphaFold2 modelling indicated that these substitution induced a conformational shift in the sidechain of arginine in the aromatic\/arginine (ar\/R) selectivity filter and in lysine at the extracellular mouth of the monomeric pore in PIP2 aquaporins. Our findings demonstrate that distal amino acid substitutions can trigger conformational changes of the ar\/R filter in the monomeric pore, modulating CO\n                    <jats:sub>2<\/jats:sub>\n                    permeability. Additionally, phylogenetic analysis suggested that aquaporins capable of dual water\/CO\n                    <jats:sub>2<\/jats:sub>\n                    permeability are ancestral to those that are water\u2010selective and CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010impermeable, and CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010selective and water impermeable.\n                  <\/jats:p>","DOI":"10.1111\/pce.15635","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T08:28:51Z","timestamp":1748939331000},"page":"6835-6846","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Amino Acid Substitutions in Loop C of Arabidopsis PIP2 Aquaporins Alters the Permeability of CO\n                    <sub>2<\/sub>"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5954-2651","authenticated-orcid":false,"given":"Shaila Shermin","family":"Tania","sequence":"first","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University Kurashiki Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeko","family":"Utsugi","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University Kurashiki Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Tsuchiya","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University Kurashiki Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizuka","family":"Sasano","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University Kurashiki Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Katsuhara","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University Kurashiki Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2235-0779","authenticated-orcid":false,"given":"Izumi C.","family":"Mori","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University Kurashiki Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1993.265.4.F463"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1140\/epje\/i2019-11912-x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12832"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.233791"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erac065"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/boc.201200057"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.70095"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2007.01757.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00033.2013"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2005.02.003"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1078220"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pch070"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04634.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-005-1399-1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcr027"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2009"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15397"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.081406"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12071511"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erab187"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PLAN.0000040900.61345.a6"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcf199"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep06665"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2006.01563.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-018-0152-0"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcf102"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-013-0548-4"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-56814-z"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18112323"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erz442"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2006-7-2-206"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00136.2006"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509469102"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.18.00453"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00008.2015"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(02)15007-8"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2009.09.002"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01488-1"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcac100"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcu003"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12410"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/bbb\/zbae092"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/35036519"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813231106"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.2.C543"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.115881"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells7110209"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcaa142"},{"key":"e_1_2_8_51_1","unstructured":"Shibasaka 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