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However, conventional systems for measuring <jats:italic>A<\/jats:italic><jats:sub>n<\/jats:sub> often require time and\/or effort to collect numerous samples in the field owing to their high weight and large size. Here, we present an efficient and convenient method for estimating <jats:italic>A<\/jats:italic><jats:sub>n<\/jats:sub> using a handheld porometer with a chlorophyll fluorometer, facilitating on\u2010the\u2010go assessment of <jats:italic>A<\/jats:italic><jats:sub>n<\/jats:sub> in the field. This porometer\u2010fluorometer method integrates the measured stomatal conductance and quantum yield of photochemistry in PSII into a biochemical photosynthesis model, incorporating model uncertainties into a single calibrated parameter. Using this method, we successfully estimated <jats:italic>A<\/jats:italic><jats:sub>n<\/jats:sub> variations in 12\u2009species under field conditions, with a root mean square error of 2.0\u2009\u03bcmol m<jats:sup>\u22122<\/jats:sup> s<jats:sup>\u22121<\/jats:sup>, despite using the common parameter set. In contrast, without calibration (i.e., with the often\u2010assumed parameter value), this method greatly overestimated <jats:italic>A<\/jats:italic><jats:sub>n<\/jats:sub>. These results highlight the importance of appropriate calibration depending on prevailing conditions, particularly the light source. In summary, this method demonstrates the potential for accessible, high\u2010throughput, and accurate estimation of <jats:italic>A<\/jats:italic><jats:sub>n<\/jats:sub> in diverse plants, thereby addressing a key bottleneck in field\u2010based phenotyping of photosynthesis. However, further studies are required to reduce the uncertainties imposed on the calibrated parameter.<\/jats:p>","DOI":"10.1111\/pce.70006","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T01:44:00Z","timestamp":1750297440000},"page":"7213-7224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Estimating Leaf CO<sub>2<\/sub> Assimilation in C<sub>3<\/sub> Plants Using a Handheld Porometer With Chlorophyll Fluorometer in Field Conditions"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6864-1563","authenticated-orcid":false,"given":"Kensuke","family":"Kimura","sequence":"first","affiliation":[{"name":"Institute for Agro\u2010Environmental Sciences National Agriculture and Food Research Organization (NARO) Tsukuba 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