{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T13:01:34Z","timestamp":1772456494575,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T00:00:00Z","timestamp":1761436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    SRC2 is a C2 domain\u2010containing protein that responds to attack from the host and non\u2010host pathogens as well as abiotic stresses. The role of SRC2 in regulating viral infection is largely unknown. We employed the BYDV\u2010barley pathosystem to characterize the involvement of HvSRC2 in viral infection. Here, we report that 17K protein encoded by barley yellow dwarf virus\u2010GAV (BYDV\u2010GAV) interacted with\n                    <jats:italic>Hordeum vulgare<\/jats:italic>\n                    SRC2 (HvSRC2) and promoted its accumulation. Silencing of HvSRC2 expression blocked BYDV\u2010GAV accumulation. Transcriptome sequencing indicated that HvSRC2 regulated plant defence\u2010related pathways like plant hormone signal transduction, plant\u2013pathogen interaction and other disease resistance pathways. Genes involved in plant immunity including\n                    <jats:italic>HvRBOHC, HvRGA1, HvB\u20102b, HvNPR2, HvBSK3, HvRPP13L3, HvMYC2<\/jats:italic>\n                    and\n                    <jats:italic>HvMkk4<\/jats:italic>\n                    were upregulated upon HvSRC2 silencing. We further found that HvRBOHC interacted with HvSRC2. HvRBOHC silencing increased BYDV\u2010GAV accumulation, coinciding with upregulated expression of other HvRBOHs and elevated H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    and O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    levels. Furthermore, we also found that MaYMV MP and TuMV viral genome\u2010linked protein (VPg), can both interact with HvSRC2, indicating the relative conservation between viral proteins and HvSRC2. Silencing of NbSRC2 inhibited the accumulation of TuMV and MaYMV. Taken together, our findings suggest that viral proteins utilize HvSRC2 to regulate plant defence pathways to aid viral infection.\n                  <\/jats:p>","DOI":"10.1111\/pce.70257","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:22:50Z","timestamp":1761542570000},"page":"760-771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Barley Yellow Dwarf Virus\u2010GAV 17K Manipulates HvSRC2 to Dampen Plant Defence to Promote Viral Infection"],"prefix":"10.1111","volume":"49","author":[{"given":"He","family":"Wang","sequence":"first","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajuan","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiongqiong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linlin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honglian","family":"Li","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"},{"name":"National Key Laboratory of Wheat and Maize Crop Science Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daowen","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agronomy Henan Agricultural University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shujie","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhumadian Academy of Agricultural Sciences Zhumadian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1263-0028","authenticated-orcid":false,"given":"Xiaoyu","family":"Han","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"},{"name":"National Key Laboratory of Wheat and Maize Crop Science Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0222"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-87836-1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/v9100294"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2025.07.004"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16772"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-11-20-0522-R"},{"issue":"7","key":"e_1_2_8_8_1","first-page":"E1282","article-title":"Harnessing Host ROS\u2010Generating Machinery for the Robust Genome Replication of a Plant RNA Virus","volume":"114","author":"Hyodo K.","year":"2017","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021110521"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba3418"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2013.06.024"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-007-0680-2"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiab451"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.05.006"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erv161"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.608541"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-phyto-121421-125135"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.15298"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15681"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2024.03.004"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13440"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.15303"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14204"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016896"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13580"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1016822"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.70281"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026468"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-009-0415-8"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adq7203"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70257","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T19:34:22Z","timestamp":1767814462000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pce.70257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,26]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/pce.70257"],"URL":"https:\/\/doi.org\/10.1111\/pce.70257","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,26]]},"assertion":[{"value":"2025-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}