{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T07:13:14Z","timestamp":1783235594289,"version":"3.54.6"},"reference-count":75,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Factors controlling plant photosynthesis and primary production include parameters dictating photosynthetic activity together with export and allocation properties. In fact, phloem loading activity as well as phloem sap redistribution and velocity are considered as important players in plant carbon acquisition. But surprisingly, there is almost no information about how phloem sap properties such as sugar concentration and velocity, are controlled by photosynthetic rate. Here, we carried out gas exchange experiments coupled to isotopic labelling on sunflower leaves to monitor photosynthate export rate when photosynthesis was varied with CO\n                    <jats:sub>2<\/jats:sub>\n                    mole fraction. We took advantage of a compartmental isotope model to obtain an experimental estimate of phloem sugar pool size and thus sap concentration and velocity. Phloem sugar concentration was found to increase relatively slowly (from very low values to about 1\u2009mol\u2009L\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    ) with photosynthesis and export rate, while sap velocity remained in the same order of magnitude (0.2\u20130.6\u2009mm\u2009s\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). This phenomenon could be explained using a simple one\u2010dimensional model of phloem sap generation and movement suggesting that at fixed photosynthesis, phloem sap was in a steady\u2010state reflecting the balance between xylem\u2010to\u2010phloem and sieve tube hydraulic conductance, turgor pressure and osmotic pressure due to loading. Our study also shows that phloem sap composition was not invariant with photosynthetic conditions but rather, adjusted to leaf export while maintaining sap flow even at very low CO\n                    <jats:sub>2<\/jats:sub>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/pce.70274","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T05:56:04Z","timestamp":1762322164000},"page":"1037-1049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Experimental Evidence for Photosynthetic Dependency of Phloem Sap Generation in Minor Veins"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3339-956X","authenticated-orcid":false,"given":"Guillaume","family":"Tcherkez","sequence":"first","affiliation":[{"name":"Research, School of Biology, ANU College of Sciences Australian National University Canberra Australia"},{"name":"Institut de recherche en horticulture et semences Universit\u00e9 d'Angers Beaucouz\u00e9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Broussard","sequence":"additional","affiliation":[{"name":"Institut de recherche en horticulture et semences Universit\u00e9 d'Angers Beaucouz\u00e9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corentin","family":"Dourmap","sequence":"additional","affiliation":[{"name":"Institut de recherche en horticulture et semences Universit\u00e9 d'Angers Beaucouz\u00e9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyril","family":"Abadie","sequence":"additional","affiliation":[{"name":"Ecophysiologie et G\u00e9nomique Fonctionnelle de la Vigne, University of Bordeaux Bordeaux science Agro, INRAE, ISVV Villenave\u2010d'Ornon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2582-1203","authenticated-orcid":false,"given":"Eric","family":"Gomes","sequence":"additional","affiliation":[{"name":"Ecophysiologie et G\u00e9nomique Fonctionnelle de la Vigne, University of Bordeaux Bordeaux science Agro, INRAE, ISVV Villenave\u2010d'Ornon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Dinant","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, INRAE, AgroParisTech Institute Jean\u2010Pierre Bourgin for Plant Sciences (IJPB) Versailles France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3966-3218","authenticated-orcid":false,"given":"Joan","family":"Doidy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Poitiers, UMR CNRS 7267 EBI Ecologie et Biologie des Interactions Poitiers 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