{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:04:43Z","timestamp":1781827483643,"version":"3.54.5"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Woody plants are fast\u2010growing and ecologically important, with widespread applications in urban landscaping and ecological restoration. However, limited understanding of the molecular mechanisms underlying adventitious shoot regeneration has hindered advances in tissue culture and genetic improvement of woody species. Here, we present a comprehensive framework for elucidating adventitious shoot regeneration in\n                    <jats:italic>Robinia pseudoacacia<\/jats:italic>\n                    . Histological analyses delineated key developmental stages, while RNA\u2010sequencing and hormone profiling highlighted the critical roles of auxin and cytokinins. WGCNA analysis identified hormone\u2010responsive regulatory modules, and chemical inhibition assays confirmed their essential functions in adventitious shoot formation. Notably, we discovered that RpARF19 and RpARR12 directly bind to the\n                    <jats:italic>RpWOX4<\/jats:italic>\n                    promoter to activate its expression and that RpWOX4, in turn, binds to the\n                    <jats:italic>RpLBD4<\/jats:italic>\n                    promoter to drive expression. In addition, RpARF19 can directly bind to the promoter of\n                    <jats:italic>RpLBD4<\/jats:italic>\n                    to activate its expression. Overexpression of\n                    <jats:italic>RpWOX4<\/jats:italic>\n                    or\n                    <jats:italic>RpLBD4<\/jats:italic>\n                    significantly enhanced adventitious shoot regeneration. Collectively, these findings establish RpWOX4 as a central integrator of auxin and cytokinins signalling and reveal a novel RpARF19\/RpARR12\u2013RpWOX4\u2013\n                    <jats:italic>RpLBD4<\/jats:italic>\n                    regulatory module that orchestrates adventitious shoot regeneration. This study provides new mechanistic insights into adventitious shoot regeneration in woody species and promising strategies for improving tissue culture and breeding approaches.\n                  <\/jats:p>","DOI":"10.1111\/pce.70411","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T03:30:09Z","timestamp":1769743809000},"page":"2706-2720","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Auxin and Cytokinins\u2010Mediated Regulation of Adventitious Shoot Regeneration via the RpARR12\/RpARF19\u2013RpWOX4\u2013\n                    <i>RpLBD4<\/i>\n                    Pathway in\n                    <i>Robinia pseudoacacia<\/i>"],"prefix":"10.1111","volume":"49","author":[{"given":"Juan","family":"Han","sequence":"first","affiliation":[{"name":"The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry Beijing Forestry University Beijing China"},{"name":"State Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, College of Biological Sciences and Technology Beijing Forestry University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shasha","family":"Ling","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, College of Biological Sciences and Technology Beijing Forestry University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanting","family":"Tian","sequence":"additional","affiliation":[{"name":"The Research Institute of Forestry and Pomology, Tianjin Academy of Agricultural Sciences Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, College of Biological Sciences and Technology Beijing Forestry University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Wu","sequence":"additional","affiliation":[{"name":"Hebei Normal University of Science &amp; Technology Qinhuangdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YiZhe","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, College of Biological Sciences and Technology Beijing Forestry University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, College of Biological Sciences and Technology Beijing Forestry University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousry A.","family":"El\u2010Kassaby","sequence":"additional","affiliation":[{"name":"Department of Forest and Conservation Sciences Faculty of Forestry The University of British Columbia Vancouver British Colombia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Deng","sequence":"additional","affiliation":[{"name":"Forestry Comprehensive Service Center of Qingshui County Natural Resources Bureau Tianshui Gansu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Forestry Comprehensive Service Center of Qingshui County Natural Resources Bureau Tianshui Gansu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Forestry Comprehensive Service Center of Qingshui County Natural Resources Bureau Tianshui Gansu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuodeng","family":"Peng","sequence":"additional","affiliation":[{"name":"The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry Beijing Forestry University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8426-565X","authenticated-orcid":false,"given":"Yun","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, College of Biological Sciences and Technology Beijing Forestry University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44319-025-00433-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.001941"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03256-2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes12030438"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.agrcom.2025.100101"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.203166"},{"key":"e_1_2_8_8_1","first-page":"232","article-title":"Chromatographic Analysis of the Flavonoids From Robinia pseudoacacia Species","volume":"39","author":"C. 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