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However, the precise mechanisms underlying the tobacco plant's response remain largely unclear. In this study, we identified a cold\u2010induced CCCH\u2010type zinc finger protein, NtZFP66L1, as an important positive regulator of cold tolerance in tobacco. Overexpressing\n                    <jats:italic>NtZFP66L1<\/jats:italic>\n                    improved cold resistance, while knocking it out made plants more sensitive to cold. NtZFP66L1 enhances cold tolerance by targeting\n                    <jats:italic>NtPP2C\u201037<\/jats:italic>\n                    and\n                    <jats:italic>NtGLK1<\/jats:italic>\n                    ; knocking out\n                    <jats:italic>NtPP2C\u201037<\/jats:italic>\n                    increased cold tolerance, while loss of\n                    <jats:italic>NtGLK1<\/jats:italic>\n                    decreased cold resistance. In plants overexpressing\n                    <jats:italic>NtZFP66L1<\/jats:italic>\n                    , abscisic acid (ABA) biosynthesis\u2010related genes, and ABA content were upregulated under both normal and cold stress conditions. Conversely, their expression and content were decreased in knockout lines. Notably, applying external ABA did not rescue the cold\u2010sensitive phenotype of\n                    <jats:italic>zfp66l1<\/jats:italic>\n                    mutants. These results suggest that NtZFP66L1 mainly influences low\u2010temperature responses through the ABA signaling pathway. Overall, our findings show that NtZFP66L1 acts as a positive regulator in cold stress by both suppressing the\n                    <jats:italic>NtPP2C\u201037<\/jats:italic>\n                    \u2010involved ABA signaling pathway and activating\n                    <jats:italic>NtGLK1<\/jats:italic>\n                    expression. This research offers valuable insights for genetically improving tobacco cultivars to enhance their ability to adapt to cold stress.\n                  <\/jats:p>","DOI":"10.1111\/pce.70545","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T09:58:02Z","timestamp":1776333482000},"page":"5328-5342","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["NtZFP66L1 Regulates Cold Tolerance in Tobacco by Modulating the ABA Signaling Pathway and the GLK1\u2010Mediated Adaptive Pathway"],"prefix":"10.1111","volume":"49","author":[{"given":"Zhengrong","family":"Hu","sequence":"first","affiliation":[{"name":"Hunan Tobacco Research Institute Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomin","family":"Ren","sequence":"additional","affiliation":[{"name":"Hunan Tobacco Research Institute Changsha Hunan 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