{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:54:05Z","timestamp":1791287645057,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Rice blast, caused by the fungal pathogen\n                    <jats:italic>Magnaporthe oryzae<\/jats:italic>\n                    , poses a severe threat to global rice production. While biological control represents a promising sustainable strategy, the molecular mechanisms by which biocontrol agents suppress\n                    <jats:italic>M. oryzae<\/jats:italic>\n                    remain poorly understood. Our findings elucidate a novel mechanism of bacterial biocontrol, wherein a secreted bacterial protease modulates fungal signalling pathways to disrupt its environmental fitness and pathogenic structures, while enhancing host resistance to blast disease. We demonstrate that the\n                    <jats:italic>Bacillus amyloliquefaciens<\/jats:italic>\n                    SN16\u20101 exhibits antagonistic activity against\n                    <jats:italic>M. oryzae<\/jats:italic>\n                    Guy11. Integrated dual RNA sequencing and protein\u2013protein interaction analysis revealed that the ergosterol biosynthesis pathway in\n                    <jats:italic>M. oryzae<\/jats:italic>\n                    is crucial for its pathogenicity and is markedly suppressed during co\u2010culture with SN16\u20101. We identified MoERG1, a key enzyme in this pathway, as essential for fungal development and virulence. Further analysis showed that the serine protease AprE, secreted by SN16\u20101, is a critical mediator. AprE triggers the Hog1\u2010MAPK signalling cascade in\n                    <jats:italic>M. oryzae<\/jats:italic>\n                    , leading to transcriptional repression of ergosterol biosynthesis genes, concomitant activation of plant disease resistance, and induction of ROS accumulation, hyperosmotic stress and apoptosis in fungal hyphae. This study provides new insights into host\u2010microbe\u2010pathogen interactions and offers an effective strategy for developing targeted biocontrol solutions against devastating plant diseases.\n                  <\/jats:p>","DOI":"10.1111\/pce.70582","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T06:07:27Z","timestamp":1778047647000},"page":"5919-5934","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protease\u2010Mediated Dual Action Against Rice Blast: Suppression of\n                    <i>Magnaporthe oryzae<\/i>\n                    Ergosterol Biosynthesis Pathway and Enhancement of Rice Disease Resistance by\n                    <i>Bacillus amyloliquefaciens<\/i>\n                    SN16\u20101"],"prefix":"10.1111","volume":"49","author":[{"given":"Xingjie","family":"He","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwei","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxu","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai 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