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Plants have evolved cross\u2010adaptation mechanisms wherein exposure to one stress enhances resistance to another. However, the specific mechanisms driving cross\u2010adaptation between saline\u2010alkali and cold stresses in alfalfa (\n                    <jats:italic>Medicago sativa<\/jats:italic>\n                    L.) remain to be elucidated. Here, we performed integrated transcriptomic and metabolomic analyses coupled with functional validation to elucidate these mechanisms. We found that saline\u2010alkali pretreatment significantly enhanced the tolerance of alfalfa to subsequent cold stress. Compared to cold stress alone, cross\u2010stress conditions increased osmolyte content and photosynthetic efficiency, while alleviating cellular oxidative damage. Integrated omics analyses revealed that cross\u2010stress specifically activated flavonoid biosynthesis, carbohydrate metabolism and abscisic acid (ABA) biosynthesis and signaling pathways. This promoted the accumulation of endogenous ABA, flavonoids, and carbohydrates. Weighted gene co\u2010expression network analysis identified\n                    <jats:italic>MsNCED3<\/jats:italic>\n                    as a critical hub gene. Exogenous ABA improved photoprotection and sugar accumulation, and enhanced cold tolerance.\n                    <jats:italic>MsNCED3<\/jats:italic>\n                    overexpression in alfalfa validated its pivotal role in cross\u2010adaptation by elevating ABA levels and mitigating oxidative damage. In conclusion, we found that\n                    <jats:italic>MsNCED3<\/jats:italic>\n                    \u2010mediated ABA accumulation, along with enhanced antioxidant and osmotic adjustment capabilities, serve as key mechanisms underlying the cross\u2010adaptation of alfalfa to saline\u2010alkali and cold stresses.\n                  <\/jats:p>","DOI":"10.1111\/pce.70603","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:02:28Z","timestamp":1778587348000},"page":"6106-6123","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["ABA Accumulation Confers Cross\u2010Adaptation to Saline\u2010Alkali and Cold Stresses in Alfalfa"],"prefix":"10.1111","volume":"49","author":[{"given":"Lei","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular and Cytogenetics, College of Life Science and Technology Harbin Normal University Harbin Heilongjiang 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College of Life Science and Technology Harbin Normal University Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juqi","family":"Chai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular and Cytogenetics, College of Life Science and Technology Harbin Normal University Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Si","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular and Cytogenetics, College of Life Science and Technology Harbin Normal University Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular and Cytogenetics, College of Life Science and Technology Harbin Normal University Harbin Heilongjiang 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