{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:54:02Z","timestamp":1791287642615,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32572785"],"award-info":[{"award-number":["32572785"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Fusarium pseudograminearum<\/jats:italic>\n                    is a soil\u2010borne phytopathogen that causes Fusarium crown rot (FCR) in wheat and barley, posing a severe threat to global grain yield and quality. A deeper understanding of its pathogenicity is essential for developing effective control strategies. Methionine (Met) has been reported that involved in plant\u2010pathogen interactions at multiple levels, regulating both plant immunity and fungal pathogenicity. Here, we identified a conserved bZIP transcription factor, FpCys3, which is a key mediator of methionine biosynthesis in\n                    <jats:italic>F. pseudograminearum<\/jats:italic>\n                    . The\n                    <jats:italic>FpCys3<\/jats:italic>\n                    mutant exhibited a series of defects, including reduced tolerance to abiotic stress, decreased conidiation, shortened conidia, reduced septa, and slowed germination rate. Specifically, deletion of\n                    <jats:italic>FpCys3<\/jats:italic>\n                    led to a significant reduction in the pathogenicity and induced the accumulation of ROS in wheat, and exogenous application of methionine could fully restore the pathogenicity of the\n                    <jats:italic>\u0394Fpcys3<\/jats:italic>\n                    mutant. We further demonstrated that FpCys3 binds to the promotors of\n                    <jats:italic>FpMet16<\/jats:italic>\n                    and\n                    <jats:italic>FpMet5<\/jats:italic>\n                    . In vegetative hyphae, FpCys3 acts as a positive regulator of these genes, and deletion of either\n                    <jats:italic>FpMet16<\/jats:italic>\n                    or\n                    <jats:italic>FpMet5<\/jats:italic>\n                    phenocopied the\n                    <jats:italic>\u0394Fpcys3<\/jats:italic>\n                    mutant, showing methionine\u2010dependent growth and reduced virulence. Despite containing a C\u2010terminus nuclear localisation signal, FpCys3 did not accumulate in the nucleus under normal conditions. However, during the early infection of\n                    <jats:italic>F. pseudograminearum<\/jats:italic>\n                    on wheat coleoptiles or under glucose treatment, FpCys3 accumulated significantly in the nucleus, where it negatively regulated the expression of\n                    <jats:italic>FpMet16<\/jats:italic>\n                    and\n                    <jats:italic>FpMet5<\/jats:italic>\n                    , and methionine synthesis. Exogenous glucose treatment markedly alleviated the necrotic lesions of wheat coleoptiles caused by\n                    <jats:italic>F. pseudograminearum<\/jats:italic>\n                    . Collectively, these findings uncover a dual role for FpCys3 in regulating methionine biosynthesis during both fungal development and host\u2010pathogen interactions, providing new insights for exploring pathogen infection mechanisms and plant disease resistance strategies.\n                  <\/jats:p>","DOI":"10.1111\/pce.70609","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T04:17:24Z","timestamp":1778559444000},"page":"6141-6154","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Dual\u2010Role Transcription Factor FpCys3 Couples Glucose Sensing to Methionine Biosynthesis and Virulence in\n                    <i>Fusarium pseudograminearum<\/i>"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4054-1277","authenticated-orcid":false,"given":"Linlin","family":"Chen","sequence":"first","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"},{"name":"State Key Laboratory of High\u2010Efficiency Production of Wheat\u2010Maize Double Cropping Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengqing","family":"Su","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guokai","family":"Duan","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"},{"name":"State Key Laboratory of High\u2010Efficiency Production of Wheat\u2010Maize Double Cropping Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Xing","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglian","family":"Li","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University Zhengzhou China"},{"name":"State Key Laboratory of High\u2010Efficiency Production of Wheat\u2010Maize Double Cropping Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"e_1_2_8_2_1","article-title":"Commodity Risk Assessment of Ligustrum Delavayanum Topiary Plants Grafted on Ligustrum Japonicum From the UK","volume":"20","author":"Bragard C.","year":"2022","journal-title":"EFSA Journal"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.6.1636S"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2007.07.005"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2010.10.002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e11-06-0532"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.210395297"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi971066i"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.849"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2021.12.007"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2018.11.007"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002952"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2019.04.013"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13912"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2011.00725.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","unstructured":"Hondorp E. 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