{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T16:21:45Z","timestamp":1785860505072,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Photosynthetic hydrogen (photoH\n                    <jats:sub>2<\/jats:sub>\n                    ) production is a promising approach for the utilisation of solar energy. Fusion of a hydrogenase (H\n                    <jats:sub>2<\/jats:sub>\n                    ase) to photosystem I (PSI) provides a direct light\u2010driven route for photoH\n                    <jats:sub>2<\/jats:sub>\n                    generation. We established the lateral transmembrane subunit PsaF of PSI as a new fusion partner for the NiFe\u2010hydrogenase (HoxYH) in the cyanobacterium\n                    <jats:italic>Synechocystis<\/jats:italic>\n                    sp. PCC 6803. Gradual reduction of PsaC expression in a PsaF\u2010HoxYH strain was established to access the most redox negative FeS\u2010cluster F\n                    <jats:sub>X<\/jats:sub>\n                    of PSI, but this did not result in photoH\n                    <jats:sub>2<\/jats:sub>\n                    production. In another approach PSI subunit PsaE was identified as a potential steric hindrance in the psaF\u2010hoxYH strain to electron transfer between the FeS\u2010cluster F\n                    <jats:sub>B<\/jats:sub>\n                    cluster in PSI and HoxYH. Consistent with this, deletion of PsaE in psaF\u2010hoxYH led to successful photoH\n                    <jats:sub>2<\/jats:sub>\n                    production. Our results identify PsaF as a viable and structurally favourable anchor providing a new strategy for the engineering of photobiological H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010production systems.\n                  <\/jats:p>","DOI":"10.1111\/pce.70659","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T03:20:58Z","timestamp":1781061658000},"page":"6850-6858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Removal of Photosystem I Subunit PsaE Leads to Photosynthetic Hydrogen Production in a Cyanobacterial PsaF\u2010HoxYH Fusion Strain"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6844-430X","authenticated-orcid":false,"given":"Florian","family":"Paul","sequence":"first","affiliation":[{"name":"Molecular Plant Physiology, Bioenergetics in Photoautotrophs University of Kassel Kassel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8562-5568","authenticated-orcid":false,"given":"Nadine","family":"Strabel","sequence":"additional","affiliation":[{"name":"Molecular Plant Physiology, Bioenergetics in Photoautotrophs University of Kassel Kassel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4049-0174","authenticated-orcid":false,"given":"Marko","family":"Boehm","sequence":"additional","affiliation":[{"name":"Molecular Plant Physiology, Bioenergetics in Photoautotrophs University of Kassel Kassel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0658-7899","authenticated-orcid":false,"given":"Jens","family":"Appel","sequence":"additional","affiliation":[{"name":"Molecular Plant Physiology, Bioenergetics in Photoautotrophs University of Kassel Kassel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4366-423X","authenticated-orcid":false,"given":"Kirstin","family":"Gutekunst","sequence":"additional","affiliation":[{"name":"Molecular Plant Physiology, Bioenergetics in Photoautotrophs University of Kassel Kassel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","unstructured":"Antonkine M. L. andJ. H.Golbeck(2006) Molecular Interactions of the Stromal Subunit PsaC with the PsaA\/PsaB Heterodimer. In Photosystem I (GOVINDJEE and Golbeck J. H. 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G.(1988) [85] Construction of Specific Mutations in Photosystem II Photosynthetic Reaction Center by Genetic Engineering Methods in Synechocystis 6803. In Cyanobacteria pp 766\u2013778 Elsevier.","DOI":"10.1016\/0076-6879(88)67088-1"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13505"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00043656"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70659","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pce.70659","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70659","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T15:25:55Z","timestamp":1785857155000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pce.70659"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,9]]},"references-count":29,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/pce.70659"],"URL":"https:\/\/doi.org\/10.1111\/pce.70659","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,9]]},"assertion":[{"value":"2026-01-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}