{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:57:50Z","timestamp":1788472670507,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Orthocaspases execute regulated cell death (RCD) in cyanobacteria, yet their post\u2010stress activation and physiological relevance remain elusive. Here, we investigated this using heterologous expression of orthocaspases in\n                    <jats:italic>Synechococcus elongatus<\/jats:italic>\n                    PCC 7942. Orthocaspase\u2010expressing (OE) strains, SynOC2_GFP and SynOC6_GFP, were generated using GFP\u2010fused orthocaspases, AnaOC2 and AnaOC6. Catalytically inactive C\/A variants were used to generate mutant\u2010expressing (ME) controls, SynC140A_GFP and SynC134A_GFP. Under non\u2010stress conditions, OE and ME strains accumulated orthocaspases without losing viability. In contrast, sulphur\u2010limitation, heat, or cold stresses accelerated cell death in OE strains. Across all treatments orthocaspases activation was consistently initiated at ~15% DNA damage and peaked beyond ~30%, temporally coincided with\n                    <jats:italic>recA<\/jats:italic>\n                    expression. RecA and orthocaspases showed direct interactions, connecting stress\u2010induced DNA damage and repair exhaustion to orthocaspases activation. Physiological relevance of the process was assessed by monitoring post\u2010stress recovery in WT co\u2010cultured with OE or ME strains. Population recovery after sulphur\u2010limitation and cold stress occurred exclusively in WT\u2009+\u2009OE co\u2010cultures. These regenerated populations were dominated by WT (85%\u201390%), indicating selective elimination of OE cells during stress. This suggested elimination of compromised cells by orthocaspases activity, limiting futile energy expenditure and propagation of mutation\u2010prone genomes to progeny. Thus, orthocaspases\u2010mediated cell death enhances inclusive fitness of cyanobacteria during abiotic stresses.\n                  <\/jats:p>","DOI":"10.1111\/pce.70685","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T07:02:13Z","timestamp":1781679733000},"page":"7004-7020","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["DNA Repair Exhaustion Activates Orthocaspase\u2010Mediated Cell Death and Promote Inclusive Fitness of Cyanobacterial Population Under Abiotic Stress"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9802-1461","authenticated-orcid":false,"given":"Samujjal","family":"Bhattacharjee","sequence":"first","affiliation":[{"name":"Laboratory of Microbial Genetics, Department of Botany, Institute of Science Banaras Hindu University Varanasi India"},{"name":"School of Biochemical Engineering, Indian Institute of Technology (IIT) Banaras Hindu University Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5131-9810","authenticated-orcid":false,"given":"Ankit","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Genetics, Department of Botany, Institute of Science Banaras Hindu University Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7517-5665","authenticated-orcid":false,"given":"Soumya Ranjan","family":"Biswal","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Genetics, Department of Botany, Institute of Science Banaras Hindu University Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7502-9574","authenticated-orcid":false,"given":"Prerna","family":"Sharma","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Genetics, Department of Botany, 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