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Here, we identified a novel ATAF subfamily transcription factor,\n                    <jats:italic>MtNACsa3,<\/jats:italic>\n                    in the closely related diploid\n                    <jats:italic>Medicago truncatula<\/jats:italic>\n                    , and elucidated its function in salt tolerance. Phenotypic analysis of the\n                    <jats:italic>MtNACsa3<\/jats:italic>\n                    mutants and overexpression lines showed its positive role in salt tolerance. Under salt or oxidative stress, MtNACsa3 relocates from the plasma membrane to the nucleus mediated by the de\u2010S\u2010acylation of Acyl\u2010Protein Thioesterase 1 (MtAPT1). MtNACsa3 up\u2010regulates the expression of\n                    <jats:italic>\n                      GDP\u2010\n                      <jats:sc>L<\/jats:sc>\n                      \u2010galactose phosphorylase 1\n                    <\/jats:italic>\n                    (\n                    <jats:italic>MtGGP1<\/jats:italic>\n                    ), enhancing ascorbic acid (AsA) synthesis to scavenge H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    . Furthermore, we created\n                    <jats:italic>MtNACsa3<\/jats:italic>\n                    exogenous transgenic alfalfa, which has higher AsA content and higher biomass than the recipient Zhongmu No.1 under salt stress. The APT1\u2013NACsa3\u2013GGP1 module not only enriches our understanding of the mechanism of salt tolerance, but also provides valuable genetic resources for salt\u2010tolerant and AsA\u2010rich alfalfa breeding.\n                  <\/jats:p>","DOI":"10.1111\/pce.70711","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T04:38:32Z","timestamp":1783053512000},"page":"7911-7926","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The APT1\u2013NACsa3\u2013GGP1 Module Enhances Salt Tolerance and Regulates Ascorbic Acid Biosynthesis in\n                    <i>Medicago<\/i>"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1269-6124","authenticated-orcid":false,"given":"Jiale","family":"Wu","sequence":"first","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-9739","authenticated-orcid":false,"given":"Jinling","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7464-2095","authenticated-orcid":false,"given":"Qinyi","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8020-7957","authenticated-orcid":false,"given":"Qianwen","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8284-718X","authenticated-orcid":false,"given":"Lihua","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7301-8442","authenticated-orcid":false,"given":"Tuo","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0099-5742","authenticated-orcid":false,"given":"Shasha","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8523-6041","authenticated-orcid":false,"given":"Shasha","family":"Ran","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3828-7919","authenticated-orcid":false,"given":"Yafei","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9878-298X","authenticated-orcid":false,"given":"Qinxi","family":"Xiao","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4001-9828","authenticated-orcid":false,"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"},{"name":"National Center of Technology Innovation for Comprehensive Utilization of Saline\u2010Alkali Land Dongying China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2643-6358","authenticated-orcid":false,"given":"Jiangli","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Biological Sciences China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.00613"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(01)00235-5"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12451"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","unstructured":"Bielen A. 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