{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T06:51:50Z","timestamp":1791442310741,"version":"4.3.3"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T00:00:00Z","timestamp":1785628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T00:00:00Z","timestamp":1785628800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32501692"],"award-info":[{"award-number":["32501692"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Angiosperm trees reorient their stems by producing tension wood (TW), a specialised xylem that generates contractile force. TW fibres develop a gelatinous (G) layer rich in cellulose, yet the molecular mechanisms governing G\u2010layer formation remain poorly understood. COBRA\u2010like genes encode glycosylphosphatidylinositol (GPI)\u2010anchored proteins known to regulate cellulose deposition. Here, we functionally characterised\n                    <jats:italic>PagCOBL5<\/jats:italic>\n                    in hybrid poplar (\n                    <jats:italic>Populus alba<\/jats:italic>\n                    \u00d7\n                    <jats:italic>P. glandulosa<\/jats:italic>\n                    ) by identifying its upstream regulator and interaction partner.\n                    <jats:italic>PagCOBL5<\/jats:italic>\n                    was preferentially expressed in mature xylem fibres, with high abundance in TW. Under mechanical stress,\n                    <jats:italic>cobl5<\/jats:italic>\n                    knockout mutants exhibited impaired negative gravitropism and significantly thinner G\u2010layer\u2014phenotypes that were reversed in\n                    <jats:italic>PagCOBL5<\/jats:italic>\n                    \u2010overexpressing lines. Furthermore, we found that the transcription factors PagLBD21 and PagLBD25 participate in the upstream negative regulation of\n                    <jats:italic>PagCOBL5<\/jats:italic>\n                    expression, with the\n                    <jats:italic>lbd21 lbd25\/+<\/jats:italic>\n                    mutant exhibiting an enhanced gravitropic response and altered xylem cell differentiation. At the protein level, PagCOBL5 directly interacted with the cellulose synthase subunits PagCESA8b. This interaction positions PagCOBL5 as a key component recruited and spatially organised by the directionally moving cellulose synthase complex (CSC). Together, these results reveal a sophisticated regulatory and protein\u2010interaction framework centred on PagCOBL5, providing pivotal molecular insights into how transcriptional modulation and cellulose synthase complexes coordinate G\u2010layer formation and mechanical force generation during the gravitropic response.\n                  <\/jats:p>","DOI":"10.1111\/pce.70773","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T05:06:59Z","timestamp":1785733619000},"page":"8898-8911","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["PagCOBL5 Integrates Transcriptional and Spatial Cues to Confer G\u2010Layer Deposition in\n                    <i>Poplar<\/i>"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6616-011X","authenticated-orcid":false,"given":"Xinwei","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Resources and Environment Qingdao Agricultural University Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongfang","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Agronomy Anhui Agricultural University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Guo","sequence":"additional","affiliation":[{"name":"Taishan Academy of Forestry Sciences Taian Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/treephys\/tpz020"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2005.02584.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12825"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13126"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1390-y"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.167270"},{"issue":"10","key":"e_1_2_8_8_1","first-page":"2800","article-title":"Transcriptional and Hormonal Regulation of Gravitropism of Woody Stems in Populus","volume":"27","author":"Gerttula S.","year":"2015","journal-title":"The Plant 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