{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T06:24:30Z","timestamp":1786947870168,"version":"build-2736575974"},"reference-count":69,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32430094"],"award-info":[{"award-number":["32430094"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31830081"],"award-info":[{"award-number":["31830081"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["JCQY201901"],"award-info":[{"award-number":["JCQY201901"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pear black spot disease (BSD) is mainly a fungal disease caused mainly\u00a0by\n                    <jats:italic>Alternaria alternata<\/jats:italic>\n                    . Although extensive research has been conducted, the precise molecular mechanisms underlying BSD resistance in pears, as well as potential genetic defence strategies, remain incompletely understood. This study investigates the role of PpHSP21, a small heat shock protein, in conferring resistance to\n                    <jats:italic>A. alternata<\/jats:italic>\n                    in pear calli. Overexpression of\n                    <jats:italic>PpHSP21<\/jats:italic>\n                    in pear calli significantly enhances\n                    <jats:italic>A. alternata<\/jats:italic>\n                    tolerance, whereas silencing the gene in pear leaves increases susceptibility. Regulation of\n                    <jats:italic>PpHSP21<\/jats:italic>\n                    involves the synergistic action of calmodulin\u2010binding protein 60B (PpCBP60B) and brassinazole\u2010resistant 1.1 (PpBZR1.1), with PpBZR1.1 also acting as a positive regulator of\n                    <jats:italic>PpCBP60B<\/jats:italic>\n                    expression. Both PpCBP60B and PpBZR1.1 directly bind to the promoter of\n                    <jats:italic>PpHSP21<\/jats:italic>\n                    , activating its expression and further confirming their roles as key transcription factors in the resistance mechanism. Additionally, pear calli overexpressing either\n                    <jats:italic>PpCBP60B<\/jats:italic>\n                    or\n                    <jats:italic>PpBZR1.1<\/jats:italic>\n                    exhibit enhanced resistance to\n                    <jats:italic>A. alternata<\/jats:italic>\n                    , while their silencing results in increased susceptibility. Moreover, we identified a functional interaction between PpHSP21 and the large ribosomal subunit protein PpRPL12, which facilitates proteostasis and increases brassinolide levels, thereby strengthening\n                    <jats:italic>A. alternata<\/jats:italic>\n                    resistance. Collectively, these findings unveil a novel regulatory module centred on PpHSP21 that possibly regulates brassinosteroid\u2010mediated tolerance to\n                    <jats:italic>A. alternata<\/jats:italic>\n                    . This study not only enhances our understanding of the molecular basis of\n                    <jats:italic>A. alternata<\/jats:italic>\n                    resistance but also provides potential genetic targets for improving crop resilience against\n                    <jats:italic>A. alternata<\/jats:italic>\n                    and other plant diseases.\n                  <\/jats:p>","DOI":"10.1111\/pce.70780","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T05:26:45Z","timestamp":1786944405000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A PpHSP21\u2010Centred Regulatory Network Integrates Brassinosteroid Signalling to Enhance Black Spot Disease Tolerance in Pear"],"prefix":"10.1111","author":[{"given":"Caihua","family":"Xing","sequence":"first","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"},{"name":"Shandong Institute of Pomology Tai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1241-4916","authenticated-orcid":false,"given":"Likun","family":"Lin","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"},{"name":"College of Horticulture Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghai","family":"Qiao","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Cheng","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Yang","sequence":"additional","affiliation":[{"name":"Hubei Academy of Agricultural Sciences Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunwei","family":"Wei","sequence":"additional","affiliation":[{"name":"Shandong Institute of Pomology Tai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Ningbo Academy of Agricultural Sciences Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Guizhou University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7479-151X","authenticated-orcid":false,"given":"Juyou","family":"Wu","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"},{"name":"Zhongshan Biological Breeding Laboratory Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Hubei Academy of Agricultural Sciences Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KeKe","family":"Zhao","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenao","family":"Gu","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huizhen","family":"Dong","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"},{"name":"Shandong Institute of Pomology Tai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyang","family":"Han","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Xie","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaijie","family":"Qi","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinmei","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Horticulture Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosan","family":"Huang","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1835-1824","authenticated-orcid":false,"given":"Shaoling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, Nanjing Agricultural University Nanjing China"},{"name":"Zhongshan Biological Breeding Laboratory Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1111\/pbi.13114","article-title":"BBX16, a B\u2010Box Protein, Positively Regulates Light\u2010Induced Anthocyanin Accumulation by Activating MYB10 in Red Pear","volume":"17","author":"Bai S.","year":"2019","journal-title":"Plant Biotechnology Journal"},{"key":"e_1_2_8_3_1","article-title":"Fire Blight of Apple and Pear: A Global Challenge for Global Fruit Production","volume":"6","author":"Bocianowski J.","year":"2026","journal-title":"JOJ Horticulture & Arboriculture"},{"key":"e_1_2_8_4_1","volume-title":"Molecular Aspects of Plant\u2010Pathogen Interaction","author":"Checker V. G.","year":"2018"},{"key":"e_1_2_8_5_1","article-title":"ERF49 Mediates Brassinosteroid Regulation of Heat Stress Tolerance in Arabidopsis thaliana","volume":"20","author":"Chen X.","year":"2022","journal-title":"BioMed Central Biology"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2025.112487"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1093\/emboj\/16.2.221","article-title":"Binding of Non\u2010Native Protein to Hsp25 During Heat Shock Creates a Reservoir of Folding Intermediates for Reactivation","volume":"16","author":"Ehrnsperger M.","year":"1997","journal-title":"EMBO Journal"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1111\/j.1365-313X.2005.02639.x","article-title":"Arabidopsis CYP90B1 Catalyses the Early C\u201022 Hydroxylation of C27, C28 and C29 Sterols","volume":"45","author":"Fujita S.","year":"2006","journal-title":"Plant Journal"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/s41477-019-0436-6","article-title":"Structural Insights Into a Key Step of Brassinosteroid Biosynthesis and Its Inhibition","volume":"5","author":"Fujiyama K.","year":"2019","journal-title":"Nature Plants,"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1126\/science.1107580","article-title":"BZR1 is a Transcriptional Repressor With Dual Roles in Brassinosteroid Homeostasis and Growth Responses","volume":"307","author":"He J. X.","year":"2005","journal-title":"Science"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.xplc.2021.100213","article-title":"Arabidopsis CALMODULIN\u2010BINDING PROTEIN 60b Plays dual Roles in Plant Immunity","volume":"2","author":"Huang W. J.","year":"2021","journal-title":"Plant Communication"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s00425-006-0361-6","article-title":"Brassinosteroid Confers Tolerance in Arabidopsis thaliana and Brassica napus to a Range of Abiotic Stresses","volume":"225","author":"Kagale S.","year":"2007","journal-title":"Planta"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s00344-003-0058-z","article-title":"Brassinosteroid\u2010Mediated Stress Responses","volume":"22","author":"Krishna P.","year":"2003","journal-title":"Journal of Plant Growth Regulation"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/30.1.325","article-title":"PlantCARE, a Database of Plant Cis\u2010Acting Regulatory Elements and a Portal to Tools for In Silico Analysis of Promoter Sequences","volume":"30","author":"Lescot M.","year":"2002","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2021.646147","article-title":"Heat Shock Protein HSP24 Is Involved in the BABA\u2010Induced Resistance to Fungal Pathogen in Postharvest Grapes Underlying an NPR1\u2010Dependent Manner","volume":"12","author":"Li C. H.","year":"2021","journal-title":"Frontiers Plant Science"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1093\/plphys\/kiab164","article-title":"Arabidopsis CBP60b Is a Central Transcriptional Activator of Immunity","volume":"186","author":"Li L. S.","year":"2021","journal-title":"Plant Physiology"},{"key":"e_1_2_8_17_1","first-page":"56","article-title":"Resistance of Different Apple Varieties Against Apple Moldy Core and Its Physiological and Biochemical Mechanism","volume":"7","author":"Li Q. L.","year":"2017","journal-title":"Journal of Agriculture"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1093\/plphys\/kiae349","article-title":"CBP60b Clade Proteins Are Prototypical Transcription Factors Mediating Immunity","volume":"196","author":"Li L. S.","year":"2024","journal-title":"Plant Physiology"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.envexpbot.2022.104782","article-title":"A WRKY Transcription Factor PbWRKY40 From Pyrus betulaefolia Functions Positively in Salt Tolerance and Modulating Organic Acid Accumulation by Regulating PbVHA\u2010B1 Expression","volume":"196","author":"Lin L.","year":"2022","journal-title":"Environmental and Experimental Botany"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16417"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2024.03.012"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1094\/PHYTO-11-18-0436-R","article-title":"Expression and Localization Patterns of a Small Heat Shock Protein That Interacts With the Helicase Domain of Cucumber Green Mottle Mosaic Virus","volume":"109","author":"Liu S. S.","year":"2019","journal-title":"Phytopathology"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real\u2010Time Quantitative PCR and the 2(\u2010Delta Delta C(T)) Method","volume":"25","author":"Livak K.","year":"2001","journal-title":"Methods"},{"key":"e_1_2_8_24_1","first-page":"9","volume-title":"Heat Shock Proteins and Plants","author":"Lopes\u2010Caitar V. S.","year":"2016"},{"key":"e_1_2_8_25_1","article-title":"The Transcriptional Regulator BZR1 Mediates Trade\u2010Off Between Plant Innate Immunity and Growth","volume":"2","author":"Lozano\u2010Dur\u00e1n R.","year":"2013","journal-title":"effe"},{"key":"e_1_2_8_26_1","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1146\/annurev-cellbio-120219-035210","article-title":"Calcium Signaling Mechanisms Across Kingdoms","volume":"37","author":"Luan S.","year":"2021","journal-title":"Annual Review of Cell and Developmental Biology"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"31033","DOI":"10.1074\/jbc.M303587200","article-title":"Refolding of Substrates Bound to Small Hsps Relies on a Disaggregation Reaction Mediated Most Efficiently by ClpB\/DnaK","volume":"278","author":"Mogk A.","year":"2003","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_8_28_1","doi-asserted-by":"crossref","first-page":"2440","DOI":"10.1111\/pce.12796","article-title":"Activation Tagging in Indica Rice Identifies Ribosomal Proteins as Potential Targets for Manipulation of Water\u2010Use Efficiency and Abiotic Stress Tolerance in Plants","volume":"39","author":"Moin M.","year":"2016","journal-title":"Plant Cell and Environment"},{"key":"e_1_2_8_29_1","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.3389\/fpls.2015.01192","article-title":"Plant Ribosomal Proteins, RPL12 and RPL19, Play a Role in Nonhost Disease Resistance Against Bacterial Pathogens","volume":"6","author":"Nagaraj S.","year":"2016","journal-title":"Frontiers in Plant Science"},{"key":"e_1_2_8_30_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s12374-023-09390-9","article-title":"Brassinosteroids and Salicylic Acid Mutually Enhance Endogenous Content and Signaling to Show a Synergistic Effect on Pathogen Resistance in Arabidopsis Thaliana","volume":"66","author":"Park C. H.","year":"2023","journal-title":"Journal of Plant Biology"},{"key":"e_1_2_8_31_1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.5423\/PPJ.RW.08.2015.0150","article-title":"Heat Shock Proteins: A Review of the Molecular Chaperones for Plant Immunity","volume":"31","author":"Park C. J.","year":"2015","journal-title":"Plant Pathology Journal"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhad261"},{"key":"e_1_2_8_33_1","unstructured":"Qian X. J.2018. \u201cMechanism of BRs in Improving Resistance toBotrytis cinereaand Drought in Tomato and Study on the Changes in Chlorophyll Fluorescence Characteristics Induced by Different Stresses.\u201d Master's thesis Zhejiang University."},{"key":"e_1_2_8_34_1","unstructured":"Qu Y.2018. \u201cStudy on the Regulation of Ethylene Synthesis in Nanguo Pear Fruit by Brassinolide.\u201d Master's thesis Shenyang Agricultural University."},{"key":"e_1_2_8_35_1","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1111\/mpp.12991","article-title":"Ribosomal Protein QM\/RPL10 Positively Regulates Defence and Protein Translation Mechanisms During Nonhost Disease Resistance","volume":"21","author":"Ramu V. S.","year":"2020","journal-title":"Molecular Plant Pathology"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2024.101072"},{"key":"e_1_2_8_37_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.plaphy.2022.07.029","article-title":"Unveiling the Functions of Plastid Ribosomal Proteins in Plant Development and Abiotic Stress Tolerance","volume":"189","author":"Robles P.","year":"2022","journal-title":"Plant Physiology and Biochemistry"},{"key":"e_1_2_8_38_1","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.molp.2019.04.003","article-title":"Two RING\u2010Finger Ubiquitin E3 Ligases Regulate the Degradation of SPX4, an Internal Phosphate Sensor, for Phosphate Homeostasis and Signaling in Rice","volume":"12","author":"Ruan W.","year":"2019","journal-title":"Molecular Plant"},{"key":"e_1_2_8_39_1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/BF00031869","article-title":"Extraction of Chlorophyll a From Freshwater Phytoplankton for Spectrophotometric Analysis","volume":"114","author":"Sartory D. P.","year":"1984","journal-title":"Hydrobiologia"},{"key":"e_1_2_8_40_1","doi-asserted-by":"crossref","DOI":"10.3390\/ijms231710149","article-title":"BES1\/BZR1 Family Transcription Factors Regulate Plant Development via Brassinosteroid\u2010Dependent and Independent Pathways","volume":"23","author":"Shi H. Y.","year":"2022","journal-title":"International Journal of Molecular Sciences"},{"key":"e_1_2_8_41_1","first-page":"487","article-title":"Transgene Expression in Pear (Pyrus communis L.) Driven by Phloem\u2010Specific Promoter AtSUC2","volume":"35","author":"Sun Q. R.","year":"2008","journal-title":"Acta Horticulturase Sinica"},{"key":"e_1_2_8_42_1","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms10159","article-title":"ChIP\u2010Seq Reveals Broad Roles of SARD1 and CBP60g in Regulating Plant Immunity","volume":"6","author":"Sun T. J.","year":"2015","journal-title":"Nature Communication"},{"key":"e_1_2_8_43_1","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1038\/ncb2151","article-title":"PP2A Activates Brassinosteroid\u2010Responsive Gene Expression and Plant Growth by Dephosphorylating BZR1","volume":"13","author":"Tang W. Q.","year":"2011","journal-title":"Nature Cell Biology"},{"key":"e_1_2_8_44_1","doi-asserted-by":"crossref","unstructured":"Tominaga A. A.Ito T.Sugiura andH.Yamane.2022.How Is Global Warming Affecting Fruit Tree Blooming? \u201cFlowering (Dormancy) Disorder\u201d in Japanese Pear (Pyrus pyrifolia) as a Case Study.\u201dFrontier in Plant Science12:787638.","DOI":"10.3389\/fpls.2021.787638"},{"key":"e_1_2_8_45_1","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1186\/1471-2229-12-216","article-title":"Co\u2010Expression Analysis Identifies Putative Targets for CBP60g and SARD1 Regulation","volume":"12","author":"Truman W.","year":"2012","journal-title":"BMC Plant Biology"},{"key":"e_1_2_8_46_1","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1104\/pp.113.227108","article-title":"The CALMODULIN\u2010BINDING PROTEIN 60 Family Includes Both Negative and Positive Regulators of Plant Immunity","volume":"163","author":"Truman W.","year":"2013","journal-title":"Plant Physiology"},{"key":"e_1_2_8_47_1","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1111\/j.1365-313X.2004.02219.x","article-title":"Visualization of Protein Interactions in Living Plant Cells Using Bimolecular Fluorescence Complementation","volume":"40","author":"Walter M.","year":"2004","journal-title":"Plant Journal"},{"key":"e_1_2_8_48_1","doi-asserted-by":"crossref","first-page":"2356","DOI":"10.1093\/plphys\/kiad208","article-title":"PbrBZR1 Interacts With PbrARI2.3 to Mediate Brassinosteroid\u2010Regulated Pollen Tube Growth During Self\u2010Incompatibility Signaling in Pear","volume":"192","author":"Wang Y.","year":"2023","journal-title":"Plant Physiology"},{"key":"e_1_2_8_49_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.plantsci.2021.111146","article-title":"Revealing the Early Response of Pear (Pyrus bretschneideri Rehd) Leaves During Botryosphaeria dothidea Infection by Transcriptome Analysis","volume":"315","author":"Wang Y.","year":"2022","journal-title":"Plant Science"},{"key":"e_1_2_8_50_1","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/S1534-5807(02)00153-3","article-title":"Nuclear\u2010Localized BZR1 Mediates Brassinosteroid\u2010Induced Growth and Feedback Suppression of Brassinosteroid Biosynthesis","volume":"2","author":"Wang Z. Y.","year":"2002","journal-title":"Developmental Cell"},{"key":"e_1_2_8_51_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000301","article-title":"Arabidopsis CaM Binding Protein CBP60g Contributes to MAMP\u2010Induced SA Accumulation and Is Involved in Disease Resistance against Pseudomonas Syringae","volume":"5","author":"Wang L.","year":"2009","journal-title":"PLOS Pathogens"},{"key":"e_1_2_8_52_1","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1111\/j.1365-313X.2011.04655.x","article-title":"CBP60g and SARD1 Play Partially Redundant Critical Roles in Salicylic Acid Signaling","volume":"67","author":"Wang L.","year":"2011","journal-title":"Plant Journal"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2024.112015"},{"key":"e_1_2_8_54_1","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1111\/nph.16536","article-title":"Plant Small Heat Shock Proteins\u2010 Evolutionary and Functional Diversity","volume":"227","author":"Waters E. R.","year":"2020","journal-title":"New Phytologist"},{"key":"e_1_2_8_55_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.154054","article-title":"Research Advances in Function and Regulation Mechanisms of Plant Small Heat Shock Proteins (sHSPs) Under Environmental Stresses","volume":"825","author":"Wu J. T.","year":"2022","journal-title":"Science of The Total Environment"},{"key":"e_1_2_8_56_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/s13059-018-1452-y","article-title":"Diversification and Independent Domestication of Asian and European Pears","volume":"19","author":"Wu J.","year":"2018","journal-title":"Genome Biology"},{"key":"e_1_2_8_57_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.plantsci.2023.111773","article-title":"PbrWRKY70 Increases Pear (Pyrus bretschneideri Rehd) Black Spot Disease Tolerance by Negatively Regulating Ethylene Synthesis via PbrERF1B\u20102","volume":"334","author":"Xing C.","year":"2023","journal-title":"Plant Science"},{"key":"e_1_2_8_58_1","first-page":"32","article-title":"Establishment and Application of Dual Cut CRISPR\/Cas9 Gene Editing and Chip\u2010Seq System in Pear Calli","author":"Yang F.","year":"2021","journal-title":"Zhejiang University"},{"issue":"10","key":"e_1_2_8_59_1","first-page":"1340","article-title":"Research Progress of Pear Black Spot and Breeding for Disease Resistance","volume":"34","author":"Yang X. P.","year":"2017","journal-title":"Journal of Fruit Science"},{"key":"e_1_2_8_60_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0135046","article-title":"Candidate Resistant Genes of Sand Pear (Pyrus pyrifolia Nakai) to Alternaria Alternata Revealed by Transcriptome Sequencing","volume":"10","author":"Yang X. P.","year":"2015","journal-title":"Plos One"},{"key":"e_1_2_8_61_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.scienta.2024.112840","article-title":"Integrated Transcriptome Sequencing and iTRAQ Quantitative Proteomics Analysis Reveal the Roles of PpJAZ1 and PpMYC2\u20102 in Sand Pear Black Spot Disease Resistance","volume":"327","author":"Yang X. P.","year":"2024","journal-title":"Scientia Horticulturae"},{"key":"e_1_2_8_62_1","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms14573","article-title":"RD26 Mediates Crosstalk Between Drought and Brassinosteroid Signalling Pathways","volume":"8","author":"Ye H. X.","year":"2016","journal-title":"Nature Communication"},{"key":"e_1_2_8_63_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.cell.2004.11.044","article-title":"A New Class of Transcription Factors Mediates Brassinosteroid\u2010Regulated Gene Expression in Arabidopsis","volume":"120","author":"Yin Y.","year":"2005","journal-title":"Cell"},{"key":"e_1_2_8_64_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-23338-y","article-title":"Structural Basis of Substrate Recognition and Thermal Protection by a Small Heat Shock Protein","volume":"12","author":"Yu C. Y.","year":"2021","journal-title":"Nature Communication"},{"key":"e_1_2_8_65_1","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1038\/nprot.2007.199","article-title":"Arabidopsis Mesophyll Protoplasts: A Versatile Cell System for Transient Gene Expression Analysis","volume":"2","author":"Yoo S. D.","year":"2007","journal-title":"Nature Protocols"},{"key":"e_1_2_8_66_1","unstructured":"Zhang R.2015. \u201cThe Research on the Mechanism ofMdWRKY33Mediated Disease Resistance Against the Apple Ring Rot Pathogenic FungiBotryosphoeria dothidea.\u201d PhD diss. Shangdong Agricultural University."},{"key":"e_1_2_8_67_1","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1038\/nprot.2006.97","article-title":"Agrobacterium\u2010Mediated Transformation of Arabidopsis Thaliana Using the Floral Dip Method","volume":"1","author":"Zhang X.","year":"2006","journal-title":"Natare Protocol"},{"key":"e_1_2_8_68_1","first-page":"18220","article-title":"Control of Salicylic Acid Synthesis and Systemic Acquired Resistance by Two Members of a Plant\u2010Specific Family of Transcription Factors","volume":"107","author":"Zhang Y. X.","year":"2010","journal-title":"Plant Biology"},{"key":"e_1_2_8_69_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1007\/s44281-023-00018-z","article-title":"Genome\u2010Wide Identification of Trihelix Transcription Factor Family Genes in Pear (Pyrus bretschneideri) and Functional Characterization of PbrGT15 in Black Spot Resistance","volume":"1","author":"Zhang Z.","year":"2023","journal-title":"Horticulture Advances"},{"issue":"6","key":"e_1_2_8_70_1","first-page":"880","article-title":"EMSA Experiments With Fluorescently Labeled Probes to Analyze the Binding Sites of Opaque 2 Protein With the ZmGRAS11 Promoter","volume":"12","author":"Zhu M. J.","year":"2022","journal-title":"Current Biotechnology"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70780","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pce.70780","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70780","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T05:26:54Z","timestamp":1786944414000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pce.70780"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,16]]},"references-count":69,"alternative-id":["10.1111\/pce.70780"],"URL":"https:\/\/doi.org\/10.1111\/pce.70780","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,16]]},"assertion":[{"value":"2025-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"pce.70780"}}