{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:23:44Z","timestamp":1787199824552,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["ZCLQ24C1401"],"award-info":[{"award-number":["ZCLQ24C1401"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo","doi-asserted-by":"publisher","award":["2024J180"],"award-info":[{"award-number":["2024J180"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32502425"],"award-info":[{"award-number":["32502425"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270146"],"award-info":[{"award-number":["32270146"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Non\u2010retroviral endogenous viral elements (nrEVEs) are viral\u2010derived sequences integrated into host genomes, but the biological functions of Chuvirus\u2010derived nrEVEs (ChuEVEs) in insects remain poorly understood. Here, we identified homologous ChuEVEs in the genomes of rice insect vectors\n                    <jats:italic>Sogatella furcifera<\/jats:italic>\n                    and\n                    <jats:italic>Laodelphax striatellus<\/jats:italic>\n                    , designated\n                    <jats:italic>SfChuEVE<\/jats:italic>\n                    and\n                    <jats:italic>LsChuEVE<\/jats:italic>\n                    .\n                    <jats:italic>SfChuEVE<\/jats:italic>\n                    and\n                    <jats:italic>LsChuEVE<\/jats:italic>\n                    showed similarity to the glycoprotein of Sanya chuvirus 2 and were detectable in most examined individuals. Transcript analysis revealed that ChuEVEs were transcriptionally active and contained predicted ORFs with distinct coding organisations. Both ChuEVEs were transcriptionally active across developmental stages and were highly expressed in salivary glands. RNAi\u2010mediated knockdown of\n                    <jats:italic>SfChuEVE<\/jats:italic>\n                    or\n                    <jats:italic>LsChuEVE<\/jats:italic>\n                    significantly prolonged nymphal development and altered genes involved in the 20\u2010hydroxyecdysone (20E) and insulin\/insulin\u2010like signalling\/target of rapamycin (IIS\/TOR) pathways in both planthopper species. Further silencing of IIS\/TOR components, including\n                    <jats:italic>S6K<\/jats:italic>\n                    and\n                    <jats:italic>FOXO<\/jats:italic>\n                    , affected selected 20E pathway\u2010related genes, particularly\n                    <jats:italic>USP<\/jats:italic>\n                    and\n                    <jats:italic>EcR<\/jats:italic>\n                    , whereas silencing IIS\/TOR components did not alter ChuEVE transcription. Moreover, exogenous 20E restored the expression of representative 20E signalling genes but failed to rescue the reduced expression of IIS\/TOR\u2010related genes following ChuEVE knockdown. Together, these findings support a role for ChuEVEs in nymphal development through an IIS\/TOR\u201320E regulatory axis. Our study provides evidence that virus\u2010derived genomic elements may acquire developmental functions after endogenisation, expanding our understanding of the functional domestication of endogenous viral elements and their potential contribution to the developmental and ecological adaptation of rice planthoppers.\n                  <\/jats:p>","DOI":"10.1111\/pce.70811","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T07:29:19Z","timestamp":1786606159000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chuvirus\u2010Derived Endogenous Viral Elements Contribute to Nymphal Development Through Modulating the IIS\/TOR \u2013 20E Pathway in Rice Planthoppers"],"prefix":"10.1111","author":[{"given":"Can","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection,Institute of Plant Virology Ningbo University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1588-8004","authenticated-orcid":false,"given":"Han\u2010Yi","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection,Institute of Plant Virology Ningbo University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuang\u2010Xin","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection,Institute of Plant Virology Ningbo University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Bin","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection,Institute of Plant Virology Ningbo University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Ping","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection,Institute of Plant Virology Ningbo University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8385-8912","authenticated-orcid":false,"given":"Jun\u2010Min","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection,Institute of Plant Virology Ningbo University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4191-9999","authenticated-orcid":false,"given":"Hong\u2010Wei","family":"Shan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection,Institute of Plant Virology Ningbo University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4968-512X","authenticated-orcid":false,"given":"Yu\u2010Juan","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection,Institute of Plant Virology Ningbo University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2012.07.007"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ve\/vez017"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2005-6-12-r99"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001030"},{"key":"e_1_2_7_6_1","first-page":"1654","article-title":"Growth Control Through Regulation of Insulin Signalling by Nutrition\u2010Activated Steroid Hormone in Drosophila","volume":"145","author":"Buhler K.","year":"2018","journal-title":"Development (Cambridge, England)"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.09.011"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2023.09.010"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00713-X"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1714590114"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.79850-0"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-4685-gmb-2022-0218"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.542437"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3199"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2017.07.021"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2022.103864"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2008.10.012"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12383"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.09.002"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08695"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02124-18"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43186-2"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03624-w"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12983-018-0290-9"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.823418"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.200373"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-08563-9"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001191"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.03091"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0507"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2008.08.003"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110397"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2018.02.001"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.72666"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00309"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2021.10.007"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-020-02141-w"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-3903-3"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.5656\/KSAE.2013.02.1.070"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.05.041"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2003.06.001"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.55446\/IJE.2021.355"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00019"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00571-17"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2021.11.005"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086675"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1603\/EC12364"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.1048208"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.09.067"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adv5257"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtherbio.2023.103626"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14286"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-56528-z"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/gix109"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70811","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pce.70811","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70811","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T03:28:15Z","timestamp":1787196495000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pce.70811"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,13]]},"references-count":53,"alternative-id":["10.1111\/pce.70811"],"URL":"https:\/\/doi.org\/10.1111\/pce.70811","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,13]]},"assertion":[{"value":"2026-06-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"pce.70811"}}