{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:02:27Z","timestamp":1789624947120,"version":"build-2803163510"},"reference-count":65,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32071814"],"award-info":[{"award-number":["32071814"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31470696"],"award-info":[{"award-number":["31470696"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Seed dormancy is a critical adaptive trait that ensures seedling establishment under favourable conditions.\n                    <jats:italic>Paeonia lactiflora<\/jats:italic>\n                    seeds exhibit dual hypocotyl and epicotyl dormancy, severely limiting large\u2010scale propagation. Although abscisic acid (ABA) is the central hormone governing dormancy, the molecular mechanisms linking ABA signalling to seed water status remain elusive. Here, we identified that the RING\u2010H2 E3 ubiquitin ligase PlSIRP1 acts as a positive regulator of ABA\u2010mediated seed dormancy in\n                    <jats:italic>P. lactiflora<\/jats:italic>\n                    .\n                    <jats:italic>PlSIRP1<\/jats:italic>\n                    expression was induced by ABA and peaked during deep dormancy. Mechanistically, PlSIRP1 promoted dormancy through dual nondegradative mechanisms: it directly bound and activated the\n                    <jats:italic>PlABI5<\/jats:italic>\n                    promoter, while concurrently interacting with PlABI5 in the nucleus to enhance its DNA\u2010binding and transcriptional repression activities without triggering PlABI5 ubiquitination or altering its stability. We further demonstrated the aquaporin\n                    <jats:italic>PlPIP2;1<\/jats:italic>\n                    as a direct downstream target; PlABI5 repressed\n                    <jats:italic>PlPIP2;1<\/jats:italic>\n                    transcription to restrict seed water uptake and hypocotyl elongation. Genetic and transgenic analyses established that PlSIRP1 functions upstream of\n                    <jats:italic>PlABI5<\/jats:italic>\n                    to maintain dormancy, a regulatory hierarchy conserved in\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    . Collectively, our findings establish the PlSIRP1\u2010PlABI5\u2010\n                    <jats:italic>PlPIP2;1<\/jats:italic>\n                    signalling module and reveal a nondegradative paradigm by which an E3 ubiquitin ligase couples ABA signalling to aquaporin\u2010mediated water transport. These results provide molecular insights into ABA\u2010dependent dormancy regulation in perennial plants and suggest potential strategies for alleviating seed dormancy to improve propagation efficiency.\n                  <\/jats:p>","DOI":"10.1111\/pce.70825","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T05:35:44Z","timestamp":1786944944000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["PlSIRP1 Activates PlABI5 via Dual Mechanisms to Repress PlPIP2;1 and Maintain ABA\u2010Mediated Seed Dormancy in\n                    <i>Paeonia lactiflora<\/i>"],"prefix":"10.1111","author":[{"given":"Rujie","family":"Xin","sequence":"first","affiliation":[{"name":"College of Forestry Shenyang Agricultural University Shenyang China"},{"name":"Key Laboratory of Edible Wild Vegetables National Forestry and Grassland Administration Shenyang China"},{"name":"Key Laboratory of Forest Tree Genetics Breeding and Cultivation of Liaoning Province Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuening","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Forestry Shenyang Agricultural University Shenyang China"},{"name":"Key Laboratory of Edible Wild Vegetables National Forestry and Grassland Administration Shenyang China"},{"name":"Key Laboratory of Forest Tree Genetics Breeding and Cultivation of Liaoning Province Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Forestry Shenyang Agricultural University Shenyang China"},{"name":"Key Laboratory of Edible Wild Vegetables National Forestry and Grassland Administration Shenyang China"},{"name":"Key Laboratory of Forest Tree Genetics Breeding and Cultivation of Liaoning Province Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghui","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Forestry Shenyang Agricultural University Shenyang China"},{"name":"Key Laboratory of Edible Wild Vegetables National Forestry and Grassland Administration Shenyang China"},{"name":"Key Laboratory of Forest Tree Genetics Breeding and Cultivation of Liaoning Province Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixin","family":"Guan","sequence":"additional","affiliation":[{"name":"College of Forestry Shenyang Agricultural University Shenyang China"},{"name":"Key Laboratory of Edible Wild Vegetables National Forestry and Grassland Administration Shenyang China"},{"name":"Key Laboratory of Forest Tree Genetics Breeding and Cultivation of Liaoning Province Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Song","sequence":"additional","affiliation":[{"name":"College of Forestry Shenyang Agricultural University Shenyang China"},{"name":"Key Laboratory of Edible Wild Vegetables National Forestry and Grassland Administration Shenyang China"},{"name":"Key Laboratory of Forest Tree Genetics Breeding and Cultivation of Liaoning Province Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimiao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Forestry Shenyang Agricultural University Shenyang China"},{"name":"Key Laboratory of Edible Wild Vegetables National Forestry and Grassland Administration Shenyang China"},{"name":"Key Laboratory of Forest Tree Genetics Breeding and Cultivation of Liaoning Province Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1782-7341","authenticated-orcid":false,"given":"Xiaomei","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Forestry Shenyang Agricultural University Shenyang China"},{"name":"Key Laboratory of Edible Wild Vegetables National Forestry and Grassland Administration Shenyang China"},{"name":"Key Laboratory of Forest Tree Genetics Breeding and Cultivation of Liaoning Province Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,16]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination","author":"Baskin C. 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