{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T12:23:40Z","timestamp":1787660620884,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The transport of manganese (Mn) from the cytosol into the vacuole is a crucial mechanism for metal and ion tolerance in plants. Mulberry exhibits potential to detoxify Mn via phytoremediation by activating the calcium\/proton exchanger 3 (\n                    <jats:italic>MaCAX3<\/jats:italic>\n                    ); however, its precise molecular mechanism remains uncharacterised. This study elucidates the molecular mechanisms by which\n                    <jats:italic>MaCAX3<\/jats:italic>\n                    confers tolerance to Mn toxicity in mulberry, demonstrating its activation via induction by methyl jasmonate (MeJA). Our findings indicate that exposure to Mn toxicity severely impaired mulberry growth and biomass, correlating with a significant decline in the maximal photochemical efficiency of PSII (\n                    <jats:italic>Fv\/Fm<\/jats:italic>\n                    ) and a reduction in chlorophyll content. Exogenous JA application, however, effectively rescued these growth and physiological parameters by enhancing Mn tolerance. The results further establish that MeJA concentrations up to 200\u2009\u03bcM released from the MeJA@ZIF\u20108 composite are pivotal in alleviating Mn toxicity, significantly boosting the activities of superoxide dismutase, peroxidase and catalase, reducing malondialdehyde content and inducing\n                    <jats:italic>MaCAX3<\/jats:italic>\n                    expression. Moreover, silencing\n                    <jats:italic>MaCAX3<\/jats:italic>\n                    compromised mulberry's Mn tolerance, whereas its overexpression in Arabidopsis enhanced Mn tolerance by diminishing reactive oxygen species through the activation of antioxidative enzymes. Collectively, these results underscore MeJA\u2010associated regulation of\n                    <jats:italic>MaCAX3<\/jats:italic>\n                    and the functional role of\n                    <jats:italic>MaCAX3<\/jats:italic>\n                    in regulating Mn and ion homoeostasis, presenting a promising target for engineering Mn phytoremediation and for developing Mn\u2010stress\u2010tolerant mulberry varieties.\n                  <\/jats:p>","DOI":"10.1111\/pce.70832","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:50:32Z","timestamp":1787316632000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Jasmonic Acid\u2010Induced Vacuolar Cation\/Proton Exchanger 3 (CAX3) Expression Drives Tolerance to Manganese Toxicity in Mulberry (\n                    <i>Morus alba<\/i>\n                    L.)"],"prefix":"10.1111","author":[{"given":"Jianbin","family":"Li","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Sericulture Biology and Biotechnology, School of Biotechnology Jiangsu University of Science and Technology Zhenjiang Jiangsu People's Republic of China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, The Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9019-8986","authenticated-orcid":false,"given":"Michael","family":"Ackah","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Sericulture Biology and Biotechnology, School of Biotechnology Jiangsu University of Science and Technology Zhenjiang Jiangsu People's Republic of China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, The Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang People's Republic of China"},{"name":"Department of Molecular Biology and Biotechnology, School of Biological Sciences, College of Agriculture and Natural Sciences University of Cape Coast Cape Coast PMB Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7910-036X","authenticated-orcid":false,"given":"Frank Kwarteng","family":"Amoako","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biotechnology, School of Biological Sciences, College of Agriculture and Natural Sciences University of Cape Coast Cape Coast PMB Ghana"},{"name":"Institute of Plant Nutrition and Soil Science Kiel University Hermann\u2010Rodewald\u2010Stra\u00dfe 2 Kiel 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