{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:13:04Z","timestamp":1788952384519,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Flooding stress is a primary environmental barrier that severely limits the widespread adoption of direct\u2010seeded rice systems. Under submerged conditions, rapid coleoptile elongation serves as a vital morphological strategy that facilitates anaerobic germination and successful seedling establishment, yet its underlying molecular mechanisms remain poorly understood. Through a genome\u2010wide association study, we identified a critical locus governing anaerobic coleoptile elongation, in which\n                    <jats:italic>OsCsLF6<\/jats:italic>\n                    , encoding a mixed\u2010linkage glucan (MLG) synthase, was characterized as the causal gene. Genetic and biochemical analyses demonstrated that\n                    <jats:italic>OsCsLF6<\/jats:italic>\n                    positively regulated coleoptile elongation by directly mediating MLG deposition into the primary cell wall. Mechanistically, we identified OsERF74, an AP2\/ERF transcription factor, as an upstream master repressor that directly binds to a conserved core\n                    <jats:italic>cis<\/jats:italic>\n                    \u2010element within the\n                    <jats:italic>OsCsLF6<\/jats:italic>\n                    promoter. Under submergence,\n                    <jats:italic>OsERF74<\/jats:italic>\n                    deficiency (\n                    <jats:italic>oserf74<\/jats:italic>\n                    mutants) completely releases this transcriptional suppression, triggering a substantial upregulation of\n                    <jats:italic>OsCsLF6<\/jats:italic>\n                    expression and subsequent hyper\u2010accumulation of cell wall MLG. In contrast, constitutive overexpression of\n                    <jats:italic>OsERF74<\/jats:italic>\n                    persistently blocks MLG biosynthesis. Crucially, a natural single\u2010nucleotide polymorphism located within the OsERF74 binding element in the promoter defines two distinct haplotypes. The elite haplotype (Hap1) effectively disrupts OsERF74 binding affinity, which in turn attenuates transcriptional repression and sustains high\n                    <jats:italic>OsCsLF6<\/jats:italic>\n                    expression, ultimately driving accelerated MLG synthesis and coleoptile elongation. Our findings establish a condition\u2010specific OsERF74\u2010OsCsLF6 regulatory module that serves as a central biochemical hub orchestrating cell wall remodelling during anaerobic germination. This module thus represents a promising molecular target and elite genetic resource for molecular breeding of flood\u2010tolerant and direct\u2010seeded rice varieties.\n                  <\/jats:p>","DOI":"10.1111\/pce.70853","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:46:13Z","timestamp":1788327973000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of\n                    <i>OsCsLF6<\/i>\n                    Gene Responsible for Rice Seed Submergence Germination Through Genome\u2010Wide Association Analysis"],"prefix":"10.1111","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"School of Life Science, Modern Agriculture Innovation Institute Hubei University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4166-4230","authenticated-orcid":false,"given":"Linfeng","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Life Science, Modern Agriculture Innovation Institute Hubei University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaya","family":"Cong","sequence":"additional","affiliation":[{"name":"School of Life Science, Modern Agriculture Innovation Institute Hubei University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7938-2181","authenticated-orcid":false,"given":"Anni","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Environmental Resilience, College of Life Sciences Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0449-2471","authenticated-orcid":false,"given":"Shiyou","family":"L\u00fc","sequence":"additional","affiliation":[{"name":"School of Life Science, Modern Agriculture Innovation Institute Hubei University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5077-8003","authenticated-orcid":false,"given":"Feng","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Life Science, Modern Agriculture Innovation Institute Hubei University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3526-4543","authenticated-orcid":false,"given":"Pingfang","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Science, Modern Agriculture Innovation Institute Hubei University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12661"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.13387"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1122975"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010146"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.15358"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00013861"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3724\/SP.J.1259.2012.00028"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad945"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2009.09.019"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0902019106"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15603"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1011052"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncrops.2024.100051"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.043000"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-005-1481-0"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201400375"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08258"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38085-5"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16376"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2015.124"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat1577"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cj.2024.11.009"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12284-023-00667-8"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1818507116"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cj.2025.09.006"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.13094"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70187"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70262"},{"key":"e_1_2_8_30_1","first-page":"299","volume-title":"Advances in Agronomy","author":"Mackill D. J.","year":"2012"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2501-8"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2007.03212.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.13122"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-013-2057-1"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcn206"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33320-x"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.195495"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants14193033"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04920"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700207"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13881"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2017.31"},{"key":"e_1_2_8_43_1","first-page":"31","article-title":"Identification of QTL Involved in Anaerobic Germination Tolerance by RIL Population in Rice","volume":"35","author":"Zhang S.","year":"2020","journal-title":"Acta Agriculturae Boreali\u2010Sinica"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70853","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pce.70853","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70853","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:29:22Z","timestamp":1788949762000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pce.70853"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":42,"alternative-id":["10.1111\/pce.70853"],"URL":"https:\/\/doi.org\/10.1111\/pce.70853","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]},"assertion":[{"value":"2026-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"pce.70853"}}