{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T09:52:16Z","timestamp":1791971536555,"version":"4.3.4"},"reference-count":77,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cold stress, including chilling and freezing, severely limits global wheat (\n                    <jats:italic>Triticum aestivum<\/jats:italic>\n                    L.) production. Although many cold\u2010responsive genes have been identified, the mechanisms that connect early cold sensing to transcriptional regulation and downstream physiological adaptation are still not fully understood. By generating\n                    <jats:italic>TaCOLD1\u20102D<\/jats:italic>\n                    overexpression and CRISPR\/Cas9 knockout lines in wheat and integrating protein interaction assays, transcriptional regulation analyses, physiological measurements, and haplotype analysis of 143 Chinese wheat accessions, we characterized the\n                    <jats:italic>TaSAP7\u2010A2\/TaCOLD1\u20102D\/TaCML5\u20107B<\/jats:italic>\n                    regulatory module.\n                    <jats:italic>TaSAP7\u2010A2<\/jats:italic>\n                    functions as a transcriptional repressor that directly binds the\n                    <jats:italic>TaCOLD1\u20102D<\/jats:italic>\n                    promoter under normal conditions. Cold exposure rapidly downregulates\n                    <jats:italic>TaSAP7\u2010A2<\/jats:italic>\n                    , relieving this repression and elevating\n                    <jats:italic>TaCOLD1\u20102D<\/jats:italic>\n                    expression. The increased\n                    <jats:italic>TaCOLD1\u20102D<\/jats:italic>\n                    then recruits TaCML5\u20107B in a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010dependent manner. This cascade activates ABA biosynthesis and signalling, promotes rapid stomatal closure, maintains ROS homoeostasis, and fine\u2010tunes\n                    <jats:italic>CBF\u2013COR<\/jats:italic>\n                    gene expression, conferring robust tolerance at both seedling and booting stages without affecting growth under non\u2010stress conditions. A natural\n                    <jats:italic>TaSAP7\u2010A2<\/jats:italic>\n                    promoter haplotype (Hap\u20107A\u20102) with weakened repressive activity shows strong artificial selection in high\u2010latitude cultivars. These results uncover an efficient derepression strategy that integrates early cold perception with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2013ABA\u2010mediated protective responses. The module provides valuable genetic targets for developing climate\u2010resilient wheat while avoiding the growth penalties associated with constitutive defence activation.\n                  <\/jats:p>","DOI":"10.1111\/pce.70866","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:46:26Z","timestamp":1789051586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cold\u2010Induced Downregulation of TaSAP7\u2010A2 Activates the TaCOLD1\u20102D\u2013TaCML5\u20107B Module to Enhance Cold Tolerance in Wheat"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7282-1942","authenticated-orcid":false,"given":"Teng","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy Northwest A&amp;F University Xianyang Shaanxi 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