{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:16:06Z","timestamp":1789049766771,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31971646"],"award-info":[{"award-number":["31971646"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["6162020"],"award-info":[{"award-number":["6162020"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Desiccation tolerance (DT) is a survival trait enabling orthodox seeds to withstand extremely low water content. While some protective factors are characterised, it remains mechanistically obscure. Here, based on PEG\u2010induced DT re\u2010establishment in germinated\n                    <jats:italic>Brassica napus<\/jats:italic>\n                    L. seeds, we investigated the dual functions of nucleotide excision repair (NER) components RAD23b and RAD4 in DNA repair and transcriptional regulation of root development. PEG pre\u2010treatment alleviated dehydration\u2010induced DNA damage and activated NER genes, suggesting the involvement of NER in seed DT. Unexpectedly, Arabidopsis\n                    <jats:italic>atrad23b<\/jats:italic>\n                    mutant and\n                    <jats:italic>BnRAD23b<\/jats:italic>\n                    \/\n                    <jats:italic>BnRAD4<\/jats:italic>\n                    over\u2010expressing seeds all exhibited significantly decreased DT after dry back, which evoked a hypothesis that BnRAD23b\u2010BnRAD4 functions beyond NER. Normally, BnRAD4 interacted with BnRAD23b and repressed the expression of root development genes\n                    <jats:italic>NAC103<\/jats:italic>\n                    ,\n                    <jats:italic>EMB1444<\/jats:italic>\n                    ,\n                    <jats:italic>RRA1<\/jats:italic>\n                    by directly binding to STRE elements within their promoters. Dehydration stress alleviated this repression, drove transcriptional reprogramming and might redirect the complex to execute DNA repair. Genetic analyses revealed that germinated seeds of\n                    <jats:italic>atnac103<\/jats:italic>\n                    ,\n                    <jats:italic>atemb1444<\/jats:italic>\n                    , and\n                    <jats:italic>atrra1<\/jats:italic>\n                    single mutants all exhibited reduced DT, and double mutants under\n                    <jats:italic>atrad23b<\/jats:italic>\n                    background almost abolished DT. This study suggests that RAD23b and RAD4 may regulate DT re\u2010establishment of germinated seeds through balancing genome integrity and radicle development, with implications for DT study broadly.\n                  <\/jats:p>","DOI":"10.1111\/pce.70867","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T13:57:33Z","timestamp":1789048653000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dual Roles of RAD23b and RAD4 on the Desiccation Tolerance of Germinated Seeds"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1466-8488","authenticated-orcid":false,"given":"Xu","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Remediation, School of Biological Sciences and Technology Beijing Forestry University Beijing 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