{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:57:40Z","timestamp":1790744260482,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Rooting depth underpins subsoil water capture and drought resilience in wheat, yet the regulators coupling root apical meristem (RAM) activity to vertical root growth remain largely unknown. We characterise\n                    <jats:italic>Deep Root Suppressor 1<\/jats:italic>\n                    (\n                    <jats:italic>TaDRS1<\/jats:italic>\n                    ;\n                    <jats:italic>TraesCS7B01G400700<\/jats:italic>\n                    ), a transcriptional target of the yield\u2010associated CONSTANS\u2010like locus\n                    <jats:italic>TaCOL\u2010B5<\/jats:italic>\n                    lying only 32\u2009kb away on chromosome 7B. In the common recessive (\n                    <jats:italic>Tacol\u2010B5<\/jats:italic>\n                    ) background of most modern wheat, chromatin immunoprecipitation\u2013qPCR and dual\u2010luciferase assays show that the Tacol\u2010B5 protein occupies a CCAAT\u2010box region of the\n                    <jats:italic>TaDRS1<\/jats:italic>\n                    promoter and activates its transcription via its conserved CCT domain. TaDRS1 is a nucleus\u2010localised protein that activates transcription when tethered to DNA, but that carries no DNA\u2010binding domain of any recognised class. In 100\u2010cm soil columns, over\u2010expression of either\n                    <jats:italic>Tacol\u2010B5<\/jats:italic>\n                    or\n                    <jats:italic>TaDRS1<\/jats:italic>\n                    confined roots to shallow horizons, whereas CRISPR\/Cas9 editing of either gene deepened rooting and increased total root length, with no detectable change in the root\u2010branching traits measured. Confocal and 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine imaging show that TaDRS1 restricts meristem size and S\u2010phase entry rather than mature cell elongation. Editing\n                    <jats:italic>TaDRS1<\/jats:italic>\n                    sustained higher leaf relative water content and preserved spike number, grain yield and thousand\u2010grain weight under drought, with no penalty under well\u2010watered conditions and no detectable change in spike length or spikelet number per spike. Mechanistically, TaDRS1 is brought to the promoter of CDK\u2010inhibitor\n                    <jats:italic>TaKRP1B<\/jats:italic>\n                    through the promoter\u2010bound C2H2 zinc\u2010finger proteins TaZFP7 and TaZFP22 and potentiates their activation of\n                    <jats:italic>TaKRP1B<\/jats:italic>\n                    . Editing\n                    <jats:italic>TaZFP7<\/jats:italic>\n                    or\n                    <jats:italic>TaZFP22<\/jats:italic>\n                    recapitulated the deep\u2010rooting phenotype of\n                    <jats:italic>TaDRS1<\/jats:italic>\n                    loss of function, and\n                    <jats:italic>TaKRP1B<\/jats:italic>\n                    over\u2010expression in the\n                    <jats:italic>TaDRS1<\/jats:italic>\n                    \u2010edited background partially suppressed it, placing TaKRP1B downstream of TaDRS1.\n                    <jats:italic>TaDRS1<\/jats:italic>\n                    is thus a candidate for uncoupling deep rooting from the linked yield locus.\n                  <\/jats:p>","DOI":"10.1111\/pce.70874","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:23:11Z","timestamp":1790742191000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Yield\u2010Associated\n                    <i>TaCOL\u2010B5<\/i>\n                    Locus Activates Its Tightly Linked Target\n                    <i>TaDRS1<\/i>\n                    to Restrict Root Apical Meristem Proliferation and Deep Rooting in Wheat"],"prefix":"10.1111","author":[{"given":"Yan","family":"Li","sequence":"first","affiliation":[{"name":"College of Science National University of Defense Technology Changsha China"},{"name":"College of Food Science and Biology Hebei University of Science and Technology Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Science National University of Defense Technology Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Germplasm Innovation and Genetic Improvement in Staple Crop, College of Agronomy Shanxi Agricultural University Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Shao","sequence":"additional","affiliation":[{"name":"College of Science National University of Defense Technology Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Li","sequence":"additional","affiliation":[{"name":"College of Science National University of Defense Technology Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9448-7902","authenticated-orcid":false,"given":"Dejing","family":"Kong","sequence":"additional","affiliation":[{"name":"College of Food Science and Biology Hebei University of Science and Technology Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6181-5156","authenticated-orcid":false,"given":"Tian","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop Sciences, Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyang","family":"Hao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop Sciences, Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linyi","family":"Qiao","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Germplasm Innovation and Genetic Improvement in Staple Crop, College of Agronomy Shanxi Agricultural University Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyu","family":"Kuang","sequence":"additional","affiliation":[{"name":"College of Science National University of Defense Technology Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2288-5981","authenticated-orcid":false,"given":"Lvyun","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Science National University of Defense Technology Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1626-0109","authenticated-orcid":false,"given":"Xiaoyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Science National University of Defense Technology Changsha China"},{"name":"State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop Sciences, Chinese Academy of Agricultural Sciences Beijing 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