{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T05:05:10Z","timestamp":1789448710067,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sugarcane yellow leaf disease, primarily caused by aphids\u2010transmitted sugarcane yellow leaf virus (SCYLV), poses a major threat to sugarcane production. The complex interactions among sugarcane, SCYLV, and aphids render phenotype\u2010based breeding inefficient for improving disease resistance. In this study, an F\n                    <jats:sub>1<\/jats:sub>\n                    \u2010population derived from a cross between the highly susceptible\n                    <jats:italic>Saccharum<\/jats:italic>\n                    spp. hybrids YT93\u2010159 and the highly resistant XTT22 were evaluated for SCYLV resistance. Thirty resistant and 20 susceptible F\n                    <jats:sub>1<\/jats:sub>\n                    \u2010individuals were selected for bulked segregant RNA\u2010seq (BSR\u2010seq). A total of 276.53\u2009Gb of clean data were generated, and 22\u2009774 single nucleotide polymorphisms (SNPs) were identified. The top five sites with the highest \u0394SNP\u2010index values above the threshold were selected from the 108 candidate regions identified by linkage analysis of genotype and phenotype. Gene annotation of these associated regions yielded 1090 candidate genes, 17 of which encoded proteins containing multiple leucine\u2010rich repeat (LRR) domains. One candidate gene encoding a protein containing 13 LRR domains was cloned and designed\n                    <jats:italic>ScLRR.<\/jats:italic>\n                    The ScLRR protein interacts with the SCYLV coat protein (SCYLV\u2010CP) in the nucleus. Furthermore, we established a method for simultaneous\n                    <jats:italic>ScLRR<\/jats:italic>\n                    overexpression and SCYLV inoculation in sugarcane roots mediated by\n                    <jats:italic>Agrobacterium rhizogenes<\/jats:italic>\n                    strain K599. Overexpression of\n                    <jats:italic>ScLRR<\/jats:italic>\n                    significantly reduced SCYLV accumulation in both sugarcane and\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    . This study provides key genetic resources and theoretical foundations for understanding the molecular mechanisms and precision breeding of sugarcane resistance to SCYLV.\n                  <\/jats:p>","DOI":"10.1111\/pce.70888","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T04:59:01Z","timestamp":1789448341000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>ScLRR<\/i>\n                    Identified From BSR\u2010seq Inhibits Sugarcane Yellow Leaf Virus Accumulation by Interacting With Its Coat Protein in the Nucleus"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8708-3365","authenticated-orcid":false,"given":"Ting","family":"Wang","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Sugarcane, College of Agriculture Fujian Agriculture and Forestry University Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9656-0801","authenticated-orcid":false,"given":"Shuai","family":"Gao","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Sugarcane, College of Agriculture Fujian Agriculture and Forestry University Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Guo","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Sugarcane, College of Agriculture Fujian Agriculture and Forestry University Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dantong","family":"Yang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Sugarcane, College of Agriculture Fujian Agriculture and Forestry University Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwu","family":"Gao","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Sugarcane, College of Agriculture Fujian Agriculture and Forestry University Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuteng","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Agri\u2010microbiomics and Biotechnology, State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3529-0268","authenticated-orcid":false,"given":"Shoujian","family":"Zang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Sugarcane, College of Agriculture Fujian Agriculture and Forestry University Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7741-4354","authenticated-orcid":false,"given":"Jian","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Agri\u2010microbiomics and Biotechnology, State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9458-112X","authenticated-orcid":false,"given":"Liping","family":"Xu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Sugarcane, College of Agriculture Fujian Agriculture and Forestry University Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s40858-019-00322-y","article-title":"Impact of Yellow Leaf Virus on Sugarcane Commercial Fields","volume":"46","author":"Asinari F.","year":"2021","journal-title":"Tropical Plant Pathology"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1007\/s11105-023-01418-1","article-title":"Uncovering Alternate Splicing Events and Premature Stop Codons Associated With Early Sucrose Accumulation in Sugarcane Using BSR\u2010Seq","volume":"43","author":"Banerjee N.","year":"2025","journal-title":"Plant Molecular Biology Reporter"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"3630","DOI":"10.1038\/s41467-025-58816-0","article-title":"A Wheat CC\u2010NBS\u2010LRR Protein Ym1 Confers WYMV Resistance by Recognizing Viral Coat 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