{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:05:46Z","timestamp":1790665546272,"version":"4.1.0"},"reference-count":105,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Lichen photobionts exhibit significant physiological variability with important ecological implications. While this variability is relatively well studied at the interspecific level, it has rarely been examined at the intraspecific level. Here, we investigated the physiological responses to temperature and irradiance of two\n                    <jats:italic>Trebouxia lynniae<\/jats:italic>\n                    strains differing by a single nucleotide polymorphism in the\n                    <jats:italic>ITS2<\/jats:italic>\n                    region. Both strains were isolated and characterized through biomass accumulation, chlorophyll fluorescence, pigment quantification, transmission electron microscopy, metabolomic profiling and whole\u2010genome sequencing. Despite sharing 99.4% and 99.9% of their nuclear and plastid genomes, respectively, the two strains displayed distinct photosynthesis performance analogous to that of the sun and shade leaves. In addition, one strain accumulated higher levels of amino acids and soluble sugars, whereas the other showed greater citrate content and cytoplasmic lipid bodies accumulation, suggesting contrasting strategies for energy storage and stress response. Genomic analysis identified over 1,600 genes carrying non\u2010synonymous mutations; interestingly, most variants were associated with genes involved in transcriptional regulation, protein turnover and signal transduction rather than core metabolic functions. These findings highlight how closely related strains can diverge functionally and point to a potential role of regulatory processes in shaping photobiont diversity and adaptation to environmental conditions.\n                  <\/jats:p>","DOI":"10.1111\/pce.70894","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:32:50Z","timestamp":1790663570000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hidden Strategies Beneath a Single Nucleotide: Contrasting Physiology in Lichen Photobiont Strains"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-8904","authenticated-orcid":false,"given":"Miguel","family":"Bl\u00e1zquez","sequence":"first","affiliation":[{"name":"Department of Mycology Real Jard\u00edn Bot\u00e1nico (CSIC) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Pizarro","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Pharmacognosy and Botany Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5421-1100","authenticated-orcid":false,"given":"Ana","family":"Garc\u00eda\u2010Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Department of Biology University Rey Juan Carlos M\u00f3stoles Spain"},{"name":"Global Change Research Institute (IICG\u2010URJC) University Rey Juan Carlos M\u00f3stoles Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5228-5368","authenticated-orcid":false,"given":"Raquel","family":"Pino\u2010Bodas","sequence":"additional","affiliation":[{"name":"Department of Biology University Rey Juan Carlos M\u00f3stoles Spain"},{"name":"Global Change Research Institute (IICG\u2010URJC) University Rey Juan Carlos M\u00f3stoles Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0266-3516","authenticated-orcid":false,"given":"Asunci\u00f3n","family":"de los R\u00edos","sequence":"additional","affiliation":[{"name":"Department of Microbial Ecology and Geomicrobiology Museo Nacional de Ciencias Naturales (CSIC) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0184-470X","authenticated-orcid":false,"given":"Miren I.","family":"Arzac","sequence":"additional","affiliation":[{"name":"Plant Ecophysiology and Irrigation (ECOVER) Group, Instituto de Recursos Naturales y Agrobiolog\u00eda de Sevilla (IRNAS\u2010CSIC) Sevilla Spain"},{"name":"Plant Ecophysiology and Irrigation (ECOVER) Group Instituto de Recursos Naturales y Agrobiolog\u00eda de Sevilla (IRNAS\u2010CSIC) Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6498-975X","authenticated-orcid":false,"given":"Jos\u00e9 Ignacio","family":"Garc\u00eda\u2010Plazaola","sequence":"additional","affiliation":[{"name":"Department of Plant Biology and Ecology University of the Basque Country Leioa Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9951-0489","authenticated-orcid":false,"given":"Beatriz","family":"Fern\u00e1ndez\u2010Mar\u00edn","sequence":"additional","affiliation":[{"name":"Department of Plant Biology and Ecology University of the Basque Country Leioa Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Unai","family":"Artetxe","sequence":"additional","affiliation":[{"name":"Department of Plant Biology and Ecology University of the Basque Country Leioa Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Toledo\u2010Gil","sequence":"additional","affiliation":[{"name":"Metabolomic Platform, CEBAS\u2010CSIC Campus Universitario Espinardo Murcia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3343-6479","authenticated-orcid":false,"given":"Fernando","family":"Vallejo","sequence":"additional","affiliation":[{"name":"Metabolomic Platform, CEBAS\u2010CSIC Campus Universitario Espinardo Murcia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo M.","family":"Casano","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Universidad de Alcal\u00e1, Alcal\u00e1 de Henares  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Israel","family":"P\u00e9rez\u2010Vargas","sequence":"additional","affiliation":[{"name":"Department of Botany, Ecology and Plant Physiology Universidad de La Laguna San Crist\u00f3bal de La Laguna Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5411-3698","authenticated-orcid":false,"given":"Sergio","family":"P\u00e9rez\u2010Ortega","sequence":"additional","affiliation":[{"name":"Department of Mycology Real Jard\u00edn Bot\u00e1nico (CSIC) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4957-4078","authenticated-orcid":false,"given":"Francisco","family":"Gasulla","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Universidad de Alcal\u00e1, Alcal\u00e1 de Henares  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.2216\/i0031-8884-6-2-127.1","article-title":"A Guide to the Algae Occurring as Lichen Symbionts: Isolation, Culture, Cultural Physiology, 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(former Trebouxia sp. TR9): biology and biogeography of an epitome lichen symbiotic microalga","volume":"11","author":"Barreno E.","year":"2022","journal-title":"Biology"},{"key":"e_1_2_8_9_1","series-title":"Lichen Biololgy","first-page":"136","volume-title":"Stress Physiology and the Symbiosis","author":"Beckett R.","year":"2008"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.phytochem.2004.12.007","article-title":"Seasonal Trends in Usnic Acid Concentrations of Arctic, Alpine and Patagonian Populations of the Lichen","volume":"66","author":"Bjerke J.","year":"2005","journal-title":"Phytochemistry"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","DOI":"10.1111\/1462-2920.70220","article-title":"Green Algal Photobiont Diversity in Lichen Communities under Forest Fragmentation","volume":"27","author":"Bl\u00e1zquez M.","year":"2025","journal-title":"Environmental Microbiology"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"4037","DOI":"10.3389\/fmicb.2021.784182","article-title":"The Role of Photobionts as Drivers of Diversification in An Island Radiation of Lichen\u2010Forming Fungi","volume":"12","author":"Bl\u00e1zquez M.","year":"2022","journal-title":"Frontiers in Microbiology"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3767\/persoonia.2024.52.03","article-title":"Endless Forms Most Frustrating: Disentangling Species Boundaries in the Ramalina decipiens Group (Lecanoromycetes, Ascomycota), With the Description of Six New Species and a Key to the Group","volume":"52","author":"Bl\u00e1zquez M.","year":"2024","journal-title":"Persoonia\u2010Molecular Phylogeny and Evolution of Fungi"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1111\/plb.70042","article-title":"Zoospore Diversity and Sexual Reproduction in the Lichen\u2010Forming Genus Trebouxia: From Neglected Evidence to New Facts","volume":"27","author":"Boccato E.","year":"2025","journal-title":"Plant Biology"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1093\/bioinformatics\/btv171","article-title":"MeDuSa: A Multi\u2010Draft Based Scaffolder","volume":"31","author":"Bosi E.","year":"2015","journal-title":"Bioinformatics"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.tplants.2004.01.006","article-title":"GABA in Plants: Just a Metabolite?","volume":"9","author":"Bouch\u00e9 N.","year":"2004","journal-title":"Trends in Plant Science"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-662-65712-6","volume-title":"Biology of Algae, Lichens and Bryophytes","author":"B\u00fcdel B.","year":"2024"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1038\/ismej.2017.119","article-title":"Exact Sequence Variants Should Replace Operational Taxonomic Units in Marker\u2010Gene Data Analysis","volume":"11","author":"Callahan B. 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