{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:01:59Z","timestamp":1790233319845,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2022YFF1302800"],"award-info":[{"award-number":["2022YFF1302800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Increasing evidence indicates that long non\u2010coding RNAs (lncRNAs) play a regulatory role in plant responses to environmental stress. However, the epigenetic mechanisms behind the regulation of salt stress by lncRNAs remain largely elusive. We previously discovered that the lncRNA\n                    <jats:italic>MtCIR2<\/jats:italic>\n                    of\n                    <jats:italic>Medicago trnucatula<\/jats:italic>\n                    negatively regulated seed germination under salt stress by altering endogenous ABA and GA concentrations via histone ubiquitination. Here we evaluated the roles of\n                    <jats:italic>MtCIR2<\/jats:italic>\n                    in regulating response of\n                    <jats:italic>M. truncatula<\/jats:italic>\n                    seedlings to salt stress. We found that over\u2010expression and mutation of\n                    <jats:italic>MtCIR2<\/jats:italic>\n                    reduced and enhanced tolerance to salt stress due to enhanced and suppressed foliar Na accumulation in over\u2010expression and\n                    <jats:italic>Mtcir2<\/jats:italic>\n                    mutant plants relative to their wild\u2010type counterparts under salt stress. The\n                    <jats:italic>MtCIR2\u2010<\/jats:italic>\n                    depenent Na accumulation was accounted for by the suppression of\n                    <jats:italic>MtSOS2<\/jats:italic>\n                    that encodes a kinase responsible for Na exclusion from plants. We discovered that\n                    <jats:italic>MtCIR2<\/jats:italic>\n                    physically interacted with BMI1, a core component of Polycomb Repressive Complex 1 (PRC1), leading to down\u2010regulation of\n                    <jats:italic>MtSOS2<\/jats:italic>\n                    . ChIP assays revealed that\n                    <jats:italic>MtCIR2<\/jats:italic>\n                    facilitated H2A ubiquitination at the chromatin of\n                    <jats:italic>MtSOS2<\/jats:italic>\n                    , thus repressing its expression, which in turn suppressed the SOS\u2010dependent Na exclusion, and rendered the plants sensitive to salt stress. These results unravel a novel mechanism by which lncRNA epigenetically regulates SOS\u2010mediated Na accumulation via histone ubiquitination in plant response to salt stress.\n                  <\/jats:p>","DOI":"10.1111\/pce.70904","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:33:08Z","timestamp":1790231588000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LncRNA\n                    <i>MtCIR2<\/i>\n                    Negatively Regulates Response of\n                    <i>Medicago truncatula<\/i>\n                    Seedlings to Salt Stress by Targeting SOS2\u2010Dependent Na Efflux via H2A Ubiquitination"],"prefix":"10.1111","author":[{"given":"Xiaohan","family":"Sun","sequence":"first","affiliation":[{"name":"Key Laboratory of Vegetation and Environmental Change, Institute of Botany Chinese Academy of Sciences Beijing China"},{"name":"College of Resources and Environment University of Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Tian","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vegetation and Environmental Change, Institute of Botany Chinese Academy of Sciences Beijing China"},{"name":"College of Resources and Environment University of Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vegetation and Environmental Change, Institute of Botany Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2708-2221","authenticated-orcid":false,"given":"Wen\u2010Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vegetation and Environmental Change, Institute of Botany Chinese Academy of Sciences Beijing China"},{"name":"College of Resources and Environment University of Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.tplants.2015.03.003","article-title":"Battles and Hijacks: Noncoding Transcription in Plants","volume":"20","author":"Ariel F.","year":"2015","journal-title":"Trends in Plant Science"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.xplc.2021.100267","article-title":"Roles of Polycomb Complexes in Regulating Gene Expression and Chromatin Structure in Plant","volume":"3","author":"Baile F.","year":"2022","journal-title":"Plant Communications"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1016\/j.cub.2010.09.046","article-title":"Keeping Cell Identity in Arabidopsis Requires PRC1 Ring\u2010Finger Homologs That Catalyze H2A Monoubiquitination","volume":"20","author":"Bratzel F.","year":"2010","journal-title":"Current Biology"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/s44319-023-00030-4","article-title":"Lncrna Dana1 Promotes Drought Tolerance and Histone Deacetylation of Drought Responsive Genes in Arabidopsis","volume":"25","author":"Cai J.","year":"2024","journal-title":"EMBO Reports"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1038\/cr.2010.151","article-title":"The Arabidopsis PRC1 \u2010Like Ring\u2010Finger Proteins Are Necessary for Repression of Embryonic Traits During Vegetative Growth","volume":"20","author":"Chen D.","year":"2010","journal-title":"Cell Research"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1093\/aob\/mcu217","article-title":"Sodium Chloride Toxicity and the Cellular Basis of Salt Tolerance in Halophytes","volume":"115","author":"Flowers T. 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