{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:01:05Z","timestamp":1790164865178,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2024YFE0214300"],"award-info":[{"award-number":["2024YFE0214300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Solanesyl diphosphate synthase (SPS) is essential for plastoquinone\u20109 (PQ\u20109) biosynthesis and has emerged as a promising target for herbicide discovery. The commercial SPS herbicides bind to the dimer interface of the protein; however, substrate\u2010competitive SPS inhibitors are still rare, particularly in agronomically relevant weed and crop orthologs. Here, we show that zoledronic acid (ZOL) inhibits\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    SPS1 with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 2.55\u2009\u03bcM in an Mg\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010dependent manner. Crystal structures of ZOL\u2010bound SPS from the weed\n                    <jats:italic>Aegilops tauschii<\/jats:italic>\n                    (\n                    <jats:italic>Ae<\/jats:italic>\n                    SPS) and the crop\n                    <jats:italic>Sorghum bicolor<\/jats:italic>\n                    (\n                    <jats:italic>Sb<\/jats:italic>\n                    SPS) revealed that the bisphosphonate moiety is anchored in the catalytic pocket through an Mg\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010water coordination network and conserved aspartate\u2010rich motifs, showing substrate\u2010competitive inhibition. ZOL induced chlorosis, root\u2010growth inhibition, and reactive oxygen species (ROS) accumulation in\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    . Transcriptomic analyses further showed that ZOL and aclonifen affected shared antioxidant\u2010related processes, but with markedly different response magnitudes. ZOL showed good herbicidal activity against\n                    <jats:italic>S. viridis<\/jats:italic>\n                    ,\n                    <jats:italic>D. sanguinalis<\/jats:italic>\n                    ,\n                    <jats:italic>V. persica<\/jats:italic>\n                    , and\n                    <jats:italic>C. album<\/jats:italic>\n                    at 1000\u2009g\u2009a.i.\/ha. Together, this work establishes ZOL as a mechanistically distinct SPS inhibitor and provides a structural basis for the development of competitive SPS\u2010inhibiting herbicides.\n                  <\/jats:p>","DOI":"10.1111\/pce.70920","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:11:17Z","timestamp":1790161877000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discovery and Structural Basis of Zoledronic Acid as a Potent SPS\u2010Targeting Herbicide"],"prefix":"10.1111","author":[{"given":"Min","family":"Li","sequence":"first","affiliation":[{"name":"International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u2010Mei","family":"Zhou","sequence":"additional","affiliation":[{"name":"International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Guo","family":"Wei","sequence":"additional","affiliation":[{"name":"International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao\u2010Jie","family":"Zeng","sequence":"additional","affiliation":[{"name":"International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi\u2010Xuan","family":"Li","sequence":"additional","affiliation":[{"name":"International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhao","sequence":"additional","affiliation":[{"name":"International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Buddhi","family":"Marambe","sequence":"additional","affiliation":[{"name":"Department of Crop Science Faculty of Agriculture, University of Peradeniya Peradeniya Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7461-1230","authenticated-orcid":false,"given":"Han","family":"Xiao","sequence":"additional","affiliation":[{"name":"International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2050-0921","authenticated-orcid":false,"given":"Da\u2010Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: A Comprehensive Python\u2010Based System for Macromolecular Structure Solution","volume":"66","author":"Adams P. D.","year":"2010","journal-title":"Acta Crystallographica. 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