{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:46:24Z","timestamp":1791996384349,"version":"4.4.0"},"reference-count":63,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100013276","name":"Interreg","doi-asserted-by":"publisher","award":["VITES QUALITAS (EFA 324\/19)"],"award-info":[{"award-number":["VITES QUALITAS (EFA 324\/19)"]}],"id":[{"id":"10.13039\/100013276","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013276","name":"Interreg","doi-asserted-by":"publisher","award":["VITRES (EFA033\/01)"],"award-info":[{"award-number":["VITRES (EFA033\/01)"]}],"id":[{"id":"10.13039\/100013276","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Perennial crops are continually wounded throughout cultivation. To limit pathogen infection and tissue dehydration, plants have evolved a range of wound responses that protect damaged tissues. These responses include changes in gene expression and the synthesis of protective compounds such as polyphenols. In addition, plants occlude damaged xylem vessels following wounding and pathogen infection, forming structures such as gels, gums, and tyloses. We explored grapevine responses to pruning. First, we examined the dynamics of xylem vessel occlusion formation at different time points and depths from the wound surface. Second, we characterised transcriptional changes and polyphenol accumulation in response to pruning. Finally, we used Raman microscopy to resolve the spatial chemical composition of xylem vessel occlusions. We found that the winter xylem vessel occlusion response is strongly associated with air temperature. Furthermore, Raman microscopy demonstrated that these occlusions were gel\u2010 or gum\u2010like structures enriched in stilbenoids and flavonoids. The accumulation of polyphenols within the occlusions was associated with the up\u2010regulation of stilbene synthase genes and the accumulation and oligomerization of stilbenes. Overall, this study provides an integrated characterisation of perennial woody tissue responses to winter pruning, combining histological, metabolomic, transcriptomic, and spectroscopic approaches, and highlights a potential regulatory role for temperature in xylem vessel occlusion formation and dynamics.<\/jats:p>","DOI":"10.1111\/pce.70921","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:37:29Z","timestamp":1790231849000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Analysis of Grapevine Responses to Winter Pruning Reveals Temperature\u2010Associated, Polyphenol\u2010Rich Xylem Occlusions"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3757-2691","authenticated-orcid":false,"given":"Marilou","family":"Cambou\u00e9","sequence":"first","affiliation":[{"name":"EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV Villenave d'Ornon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6240-8605","authenticated-orcid":false,"given":"Monica","family":"Galar\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Biotechnology and Food Science Univ. P\u00fablica de Navarra UPNA Pamplona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2411-7428","authenticated-orcid":false,"given":"Gr\u00e9goire","family":"Loupit","sequence":"additional","affiliation":[{"name":"Ume\u00e5 Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences Ume\u00e5 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5034-071X","authenticated-orcid":false,"given":"Paraskevi","family":"Charalambous","sequence":"additional","affiliation":[{"name":"Institute of Biophysics BOKU University Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enric","family":"Zehraoui","sequence":"additional","affiliation":[{"name":"EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV Villenave d'Ornon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4624-0363","authenticated-orcid":false,"given":"Joseph","family":"Tran","sequence":"additional","affiliation":[{"name":"EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV Villenave d'Ornon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1359-4114","authenticated-orcid":false,"given":"Josep Valls","family":"Fonayet","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV Villenave\u2010d'Ornon France"},{"name":"Bordeaux Metabolome Facility, MetaboHUB, PHENOME\u2010EMPHASIS Villenave d'Ornon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6924-6744","authenticated-orcid":false,"given":"Luis Gonzaga","family":"Santesteban","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Biotechnology and Food Science Univ. P\u00fablica de Navarra UPNA Pamplona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3699-9931","authenticated-orcid":false,"given":"Notburga","family":"Gierlinger","sequence":"additional","affiliation":[{"name":"Institute of Biophysics BOKU University Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2591-192X","authenticated-orcid":false,"given":"Sarah Jane","family":"Cookson","sequence":"additional","affiliation":[{"name":"EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV Villenave d'Ornon France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-083023-034055"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2025.109806"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1609-3_9"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13434"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44297-025-00055-2"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-017-1100-5"},{"key":"e_1_2_7_8_1","first-page":"165","volume-title":"Sustainable Grape and Wine Production in the Context of Climate Change \u2010 ClimWIne 2016","author":"Destrac Irvine A.","year":"2016"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants9060780"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-021-03177-y"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/f13081258"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0439-x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dendro.2013.07.002"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz931"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.066993"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-04-17-0512-FE"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-018-0046-7"},{"key":"e_1_2_7_18_1","unstructured":"Hartig F.2024. DHARMa: Residual Diagnostics for Hierarchical (Multi\u2010Level\/Mixed) Regression Models. R package version 0.4.7."},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.18.00293"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01035"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438-021-00585-0"},{"key":"e_1_2_7_22_1","unstructured":"Kassambara A. andF.Mundt2020. Factoextra: Extract and Visualize the Results of Multivariate Data Analyses. R Package Version 1.0.7.https:\/\/CRAN.R-project.org\/package=factoextra."},{"key":"e_1_2_7_23_1","unstructured":"Kolde R.2025. pheatmap: Pretty Heatmaps. R Package Version 1.0.13.https:\/\/github.com\/raivokolde\/pheatmap."},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.16.00490"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v025.i01"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-82848-3"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14693"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b08099"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.70244"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-022-03821-4"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erag002"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c09502"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0621-6_15"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1163\/22941932-20160130"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2023.108900"},{"key":"e_1_2_7_36_1","unstructured":"Oksanen J. G.Simpson F.Blanchet et al.2026. vegan: Community Ecology Package. R Package Version 2.8\u20100.https:\/\/vegandevs.github.io\/vegan\/."},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.agrformet.2013.06.005"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiae203"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11121744"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00253"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/treephys\/tpz036"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.1998.88.6.494"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00470"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2019.01.003"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.0800061"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.100537"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/ffgc.2019.00018"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3820"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcy045"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx192"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkad067"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.00234"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2023.112741"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erz430"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsw028"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2019.110950"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2024.09.003"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12162901"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes14020464"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants7010007"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2024.119941"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10725-013-9838-z"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70921","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pce.70921","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70921","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:03:10Z","timestamp":1791993790000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pce.70921"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"references-count":63,"alternative-id":["10.1111\/pce.70921"],"URL":"https:\/\/doi.org\/10.1111\/pce.70921","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]},"assertion":[{"value":"2026-06-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"pce.70921"}}