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RNAi is promising but faces challenges including environmental instability of dsRNA and poor cellular delivery efficiency. In this study, we engineered the cotton rhizobacterium\n                    <jats:italic>Pantoea agglomerans<\/jats:italic>\n                    YC4 to deliver dsRNA into\n                    <jats:italic>Verticillium dahliae<\/jats:italic>\n                    . Small RNA\u2010seq revealed that bacterial outer membrane vesicles (OMVs) selectively enrich specific endogenous sRNAs compared with intracellular compartments, with a congruent sRNA enrichment pattern observed in fungal cells, thus confirming OMVs as the primary carriers for cross\u2010kingdom RNA delivery. Synthetic biology strategies, including promoter engineering and OmpA\u2010DRB4 fusion, further boosted dsRNA production and OMVs loading capacity. Greenhouse trials demonstrated the persistent colonization of engineered strain in the cotton rhizosphere, and alleviated Verticillium wilt symptoms and reduced fungal burden. This work establishes an engineered rhizosphere symbiont platform for\n                    <jats:italic>in situ<\/jats:italic>\n                    RNAi delivery, offering a practical and sustainable strategy for controlling soil\u2010borne fungal diseases.\n                  <\/jats:p>","DOI":"10.1111\/pce.70939","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:00:51Z","timestamp":1790686851000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineered Rhizobacteria Exploit Bacterial Vesicles for Cross\u2010Kingdom RNAi and Soilborne Disease Control"],"prefix":"10.1111","author":[{"given":"Haifeng","family":"Ding","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering Shihezi University Shihezi China"},{"name":"State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, School of Chemistry and Chemical Engineering Shihezi University Shihezi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Qiu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering Shihezi University Shihezi China"},{"name":"State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, School of Chemistry and Chemical Engineering Shihezi University Shihezi China"},{"name":"Key Lab of Process Analysis and Control of Sichuan Universities Yibin University Yibin P.R. 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