{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:52:17Z","timestamp":1791183137950,"version":"4.1.0"},"reference-count":78,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp;amp; Environment"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Nitro\u2010fatty acids (NO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010FAs) are potent electrophiles that react with nucleophilic residues in proteins (cysteines, histidines and lysines) via a process known as nitroalkylation. This reversible post\u2010translational modification (PTM) functions as a selective signalling mechanism by modulating the structure, transport, and catalysis of target proteins. In the present work,\n                    <jats:italic>in vivo<\/jats:italic>\n                    nitroalkylated proteins targets by nitro\u2010oleic acid (NO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010OA), nitro\u2010linoleic acid (NO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010LA) and NO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010Ln have been identified in different Arabidopsis development, including the seed, vegetative, generative and senescence stages. The results reveal that nitroalkylation is a PTM that occurs throughout the development of Arabidopsis, primarily during the vegetative and generative stages. This modification predominantly affects proteins involved in energy metabolism, redox coenzymes and stress responses. Although all three NO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010FAs interact with the three amino acids mentioned, cysteine is the primary target. However, its adducts are significantly more labile and redox\u2010sensitive than those formed with histidine or lysine. Overall, these results suggest that nitroalkylation constitutes a reversible post\u2010translational modification linked to the redox state. This regulation could participate in modulating the plasticity of energy flow, contributing to maintaining homoeostasis throughout the life cycle and resilience to environmental stress. Data are available via ProteomeXchange with identifier PXD060471.\n                  <\/jats:p>","DOI":"10.1111\/pce.70956","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:24:58Z","timestamp":1791181498000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nitroalkylation Mediated by Nitro\u2010Fatty Acids Is Involved in Key Physiological Processes During\n                    <i>Arabidopsis thaliana<\/i>\n                    Development"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0460-8075","authenticated-orcid":false,"given":"Lorena","family":"Aranda\u2010Ca\u00f1o","sequence":"first","affiliation":[{"name":"Group of Biochemistry and Cell Signalling in Nitric Oxide University Institute of Research on Olive and Olive Oils, University of Ja\u00e9n Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2253-0101","authenticated-orcid":false,"given":"Raquel","family":"Valderrama","sequence":"additional","affiliation":[{"name":"Group of Biochemistry and Cell Signalling in Nitric Oxide University Institute of Research on Olive and Olive Oils, University of Ja\u00e9n Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8393-7012","authenticated-orcid":false,"given":"Mounira","family":"Chaki","sequence":"additional","affiliation":[{"name":"Group of Biochemistry and Cell Signalling in Nitric Oxide University Institute of Research on Olive and Olive Oils, University of Ja\u00e9n Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9017-4597","authenticated-orcid":false,"given":"Juan Carlos","family":"Begara\u2010Morales","sequence":"additional","affiliation":[{"name":"Group of Biochemistry and Cell Signalling in Nitric Oxide University Institute of Research on Olive and Olive Oils, University of Ja\u00e9n Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-0638","authenticated-orcid":false,"given":"Jos\u00e9 Rafael","family":"Pedrajas","sequence":"additional","affiliation":[{"name":"Group of Biochemistry and Cell Signalling in Nitric Oxide University Institute of Research on Olive and Olive Oils, University of Ja\u00e9n Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2210-9547","authenticated-orcid":false,"given":"Manuel","family":"Melguizo","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Organic Chemistry University of Ja\u00e9n Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7809-9800","authenticated-orcid":false,"given":"Mat\u00edn","family":"Moret","sequence":"additional","affiliation":[{"name":"Group of Transcription and Gene Expression in Eukaryotes University Institute of Research on Olive and Olive Oils, University of Ja\u00e9n Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6257-8914","authenticated-orcid":false,"given":"Aureliano","family":"Bombarely","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology of Plants Spanish National Research Council\u2010Universitat Polit\u00e8cnica de Val\u00e8ncia (IBMCP\u2010CSIC\u2010UPV) Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1354-3533","authenticated-orcid":false,"given":"Francisco","family":"Luque","sequence":"additional","affiliation":[{"name":"Group of Transcription and Gene Expression in Eukaryotes University Institute of Research on Olive and Olive Oils, University of Ja\u00e9n Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9477-9195","authenticated-orcid":false,"given":"Juan Bautista","family":"Barroso","sequence":"additional","affiliation":[{"name":"Group of Biochemistry and Cell Signalling in Nitric Oxide University Institute of Research on Olive and Olive Oils, University of Ja\u00e9n Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants8040082"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11101869"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11050972"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1157956"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2208924120"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2006.01.005"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704085200"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.11.021"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M602814200"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.21.4319"},{"key":"e_1_2_11_12_1","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4899-1002-8","volume-title":"Seeds: Physiology of Development and Germination","author":"Bewley J. D.","year":"1994"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010011"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-6141403\/v1"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2019.110243"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02177.x"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.70076"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erp263"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/ssp073"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbc.2024.100037"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.031591"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2015.07.149"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-4685-GMB-2016-0015"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-012-1675-1"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102126"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.033589"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R800004200"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01944"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2011.06.014"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-002-0913-3"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20111752"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar900286y"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.7.3.309"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2018.11.008"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx287"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.003566"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26247536"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008993"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.1991.tb04895.x"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.11.2692"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erac462"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01144"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err042"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.692108"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcu072"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00962"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.15.01671"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2010.02133.x"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912149107"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2018.03.016"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-043014-115627"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2022.07.025"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2646"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/membranes12060606"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2016.12.009"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1011"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2024.09.009"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.290re7"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.109.201582"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr200131e"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2019.04.009"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.01884"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.052423"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0903478106"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.pcp.a029329"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx382"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eraa183"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.161612"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2018.03.014"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.314971"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24747"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1023358"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants9030406"},{"key":"e_1_2_11_74_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.redox.2021.101987","article-title":"Electrophilic Nitro\u2010Fatty Acids Suppress Psoriasiform Dermatitis: STAT3 Inhibition as a Contributory Mechanism","volume":"43","author":"Wang P.","year":"2021","journal-title":"Redox Biology"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.101987"},{"key":"e_1_2_11_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2023.105590"},{"key":"e_1_2_11_77_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00321.2015"},{"key":"e_1_2_11_78_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.814368"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2014.10.003"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70956","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pce.70956","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.70956","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:24:59Z","timestamp":1791181499000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pce.70956"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,4]]},"references-count":78,"alternative-id":["10.1111\/pce.70956"],"URL":"https:\/\/doi.org\/10.1111\/pce.70956","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,4]]},"assertion":[{"value":"2026-05-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"pce.70956"}}