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Here, we performed genome\u2010wide <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation analysis of primary cutaneous melanoma and benign melanocytic nevus interrogating 14\u00a0495 genes using BeadChip technology. This genome\u2010wide view of promoter methylation in primary cutaneous melanoma revealed an array of recurrent <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation alterations with potential diagnostic applications. Among 106 frequently hypermethylated genes, there were many novel methylation targets and tumor suppressor genes. Highly recurrent methylation of the <jats:italic><jats:styled-content style=\"fixed-case\">HOXA<\/jats:styled-content>9<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>13<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">CDH<\/jats:styled-content>11<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">PLEKHG<\/jats:styled-content>6<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">PPP<\/jats:styled-content>1R3C,<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">CLDN<\/jats:styled-content>11<\/jats:italic> genes was established. Promoter methylation of <jats:italic><jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>13<\/jats:italic>, encoding p38\u03b4, was present in 67% of primary and 85% of metastatic melanomas. Restoration of <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>13 expression in melanoma cells exhibiting epigenetic silencing of this gene reduced proliferation, indicative of tumor suppressive functions. This study demonstrates that <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation alterations are widespread in melanoma and suggests that epigenetic silencing of <jats:italic><jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>13<\/jats:italic> contributes to melanoma progression.<\/jats:p>","DOI":"10.1111\/pcmr.12096","type":"journal-article","created":{"date-parts":[[2013,3,28]],"date-time":"2013-03-28T10:16:22Z","timestamp":1364465782000},"page":"542-554","source":"Crossref","is-referenced-by-count":55,"title":["Genome\u2010wide promoter methylation analysis identifies epigenetic silencing of <scp><i>MAPK<\/i><\/scp><i>13<\/i> in primary cutaneous melanoma"],"prefix":"10.1111","volume":"26","author":[{"given":"Linda","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjon A.","family":"Smit","sequence":"additional","affiliation":[{"name":"Division of Molecular Oncology The Netherlands Cancer Institute Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost J.","family":"van den Oord","sequence":"additional","affiliation":[{"name":"Department of Pathology KU Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelle J.","family":"Goeman","sequence":"additional","affiliation":[{"name":"Department of Medical Statistics Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Els M. 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