{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:07:22Z","timestamp":1790381242456,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,5,13]],"date-time":"2013-05-13T00:00:00Z","timestamp":1368403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aim of present study was to evaluate CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup> ratio and CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>hi<\/jats:sup>FoxP3<jats:sup>+<\/jats:sup> Tregs in GV patients with reference to their effect on disease onset and progression. Flow cytometry was used for determination of CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup> ratio and Tregs in 82 patients and 50 controls. CD8<jats:sup>+<\/jats:sup> T\u2010cell counts were significantly higher in GV patients as compared with controls (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.003). Active GV patients showed higher CD8<jats:sup>+<\/jats:sup> T\u2010cell counts compared with stable GV patients (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.001). The CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup> ratio decreased significantly in patients as compared with controls (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.001). Moreover, the ratio in active GV patients significantly lowered as compared with stable GV patients (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.002). Significant decrease in Treg cell percentage and counts in GV patients was observed compared with controls (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.009, p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.008) with significant reduction in FoxP3 expression (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.024). Treg cell percentage and counts were significantly decreased in active GV patients compared with stable GV patients (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.007, p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.002). Our results suggest that an imbalance of CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup> ratio and natural Tregs in frequency and function might be involved in the T\u2010cell mediated pathogenesis of GV and its progression.<\/jats:p>","DOI":"10.1111\/pcmr.12105","type":"journal-article","created":{"date-parts":[[2013,4,10]],"date-time":"2013-04-10T10:10:54Z","timestamp":1365588654000},"page":"586-591","source":"Crossref","is-referenced-by-count":145,"title":["Decreased regulatory <scp>T<\/scp>\u2010cells and <scp>CD<\/scp>4<sup>+<\/sup>\/<scp>CD<\/scp>8<sup>+<\/sup> ratio correlate with disease onset and progression in patients with generalized vitiligo"],"prefix":"10.1111","volume":"26","author":[{"given":"Mitesh","family":"Dwivedi","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naresh C.","family":"Laddha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prateek","family":"Arora","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yogesh S.","family":"Marfatia","sequence":"additional","affiliation":[{"name":"Department of Skin and VD Sir Sayajirao Gaikwad Medical College, The Maharaja Sayajirao University of Baroda Vadodara India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasheedunnisa","family":"Begum","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,13]]},"reference":[{"key":"e_1_2_4_2_1","first-page":"117","article-title":"Evaluation of circulating CD4\u00a0+\u00a0 CD25high FoxP3\u00a0+\u00a0 T lymphocytes in active non\u2010segmental vitiligo","volume":"20","author":"Abdallah M.","year":"2009","journal-title":"J. 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