{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:27:00Z","timestamp":1784827620977,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,17]],"date-time":"2014-01-17T00:00:00Z","timestamp":1389916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We characterized a zebrafish mutant that displays defects in melanin synthesis and in the differentiation of melanophores and iridophores of the skin and retinal pigment epithelium. Positional cloning and candidate gene sequencing link this mutation to a 410\u2010kb region on chromosome 6, containing the <jats:italic>oculocutaneous albinism 2<\/jats:italic> (<jats:italic>oca2<\/jats:italic>) gene. Quantification of <jats:italic>oca2<\/jats:italic> mutant melanophores shows a reduction in the number of differentiated melanophores compared with wildtype siblings. Consistent with the analysis of mouse <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>ca2\u2010deficient melanocytes, zebrafish mutant melanophores have immature melanosomes which are partially rescued following treatment with vacuolar\u2010type <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>ase inhibitor\/cytoplasmic p<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> modifier, bafilomycin <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>1. Melanophore\u2010specific gene expression is detected at the correct time and in anticipated locations. While <jats:italic>oca2<\/jats:italic> zebrafish display unpigmented gaps on the head region of mutants 3\u00a0days post\u2010fertilization, melanoblast quantification indicates that <jats:italic>oca2<\/jats:italic> mutants have the correct number of melanoblasts, suggesting a differentiation defect explains the reduced melanophore number. Unlike melanophores, which are reduced in number in <jats:italic>oca2<\/jats:italic> mutants, differentiated iridophores are present at significantly higher numbers. These data suggest distinct mechanisms for <jats:italic>oca2<\/jats:italic> in establishing differentiated chromatophore number in developing zebrafish.<\/jats:p>","DOI":"10.1111\/pcmr.12205","type":"journal-article","created":{"date-parts":[[2013,12,11]],"date-time":"2013-12-11T18:08:55Z","timestamp":1386785335000},"page":"178-189","source":"Crossref","is-referenced-by-count":58,"title":["<i>oca2<\/i> regulation of chromatophore differentiation and number is cell type specific in zebrafish"],"prefix":"10.1111","volume":"27","author":[{"given":"Alisha J.","family":"Beirl","sequence":"first","affiliation":[{"name":"School of Molecular Biosciences Washington State University Vancouver Vancouver WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tor H.","family":"Linbo","sequence":"additional","affiliation":[{"name":"Department of Biological Structure University of Washington Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marea J.","family":"Cobb","sequence":"additional","affiliation":[{"name":"School of Molecular Biosciences Washington State University Vancouver Vancouver WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia D.","family":"Cooper","sequence":"additional","affiliation":[{"name":"School of Molecular Biosciences Washington State University Vancouver Vancouver WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01795-6"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700683"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2009.00614.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2007.00226.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2001.140203.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.02-02-0022"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1600-0749.2003.00106.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065096"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00598"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.030494"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2003.014902"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-2-43"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12111"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2010.00815.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1212821"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020197"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(98)00176-1"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1093\/genetics\/141.4.1563","article-title":"Molecular analysis of 36 mutations at the mouse pink\u2010eyed dilution (p) locus","volume":"141","author":"Johnson D.K.","year":"1995","journal-title":"Genetics"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9840"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.17.3757"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2001.140508.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.2001.1006"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.12.115"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.2006.00345.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2005.08.013"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1700"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00108.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1997.3901"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2010.06.002"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1999.5824"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E08-07-0710"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2007.12.008"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119.4.1203"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.040667"},{"key":"e_1_2_7_37_1","volume-title":"The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio)","author":"Westerfield M.","year":"2000"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1247\/csf.23.33"}],"container-title":["Pigment Cell &amp; Melanoma Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpcmr.12205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pcmr.12205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T13:54:39Z","timestamp":1695563679000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pcmr.12205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,17]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1111\/pcmr.12205"],"URL":"https:\/\/doi.org\/10.1111\/pcmr.12205","archive":["Portico"],"relation":{},"ISSN":["1755-1471","1755-148X"],"issn-type":[{"value":"1755-1471","type":"print"},{"value":"1755-148X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,17]]}}}