{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T01:07:49Z","timestamp":1789952869283,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,7,14]],"date-time":"2014-07-14T00:00:00Z","timestamp":1405296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R33 CA160138, R21 CA134368, R01 CA112243, and P30 CA016086."],"award-info":[{"award-number":["R33 CA160138, R21 CA134368, R01 CA112243, and P30 CA016086."]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation studies have elucidated a methylation signature distinguishing primary melanomas from benign nevi and provided new insights about genes that may be important in melanoma development. However, it is unclear whether methylation differences among primary melanomas are related to tumor pathologic features with known clinical significance. We utilized the Illumina GoldenGate Cancer Panel array to investigate the methylation profiles of 47 primary cutaneous melanomas. Arraywide methylation patterns revealed a positive association of methylation with Breslow thickness and mutated <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic>, a negative association with mitotic rate, and a weak association with ulceration. Hierarchical clustering on CpG sites exhibiting the most variable methylation (n\u00a0=\u00a0235) divided the melanoma samples into three clusters, including a highly methylated cluster that was positively associated with Breslow thickness and an intermediately methylated cluster associated with Breslow thickness and mitotic rate. Our findings provide support for the existence of methylation\u2010defined subsets in melanomas with increased methylation associated with Breslow thickness.<\/jats:p>","DOI":"10.1111\/pcmr.12289","type":"journal-article","created":{"date-parts":[[2014,7,2]],"date-time":"2014-07-02T00:27:17Z","timestamp":1404260837000},"page":"1097-1105","source":"Crossref","is-referenced-by-count":24,"title":["<scp>DNA<\/scp> methylation profiles in primary cutaneous melanomas are associated with clinically significant pathologic features"],"prefix":"10.1111","volume":"27","author":[{"given":"Nancy E.","family":"Thomas","sequence":"first","affiliation":[{"name":"Department of Dermatology University of North Carolina Chapel Hill NC USA"},{"name":"Lineberger Comprehensive Cancer Center University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel A.","family":"Slater","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon N.","family":"Edmiston","sequence":"additional","affiliation":[{"name":"Lineberger Comprehensive Cancer Center University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Biostatistics University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Fen","family":"Kuan","sequence":"additional","affiliation":[{"name":"Lineberger Comprehensive Cancer Center University of North Carolina Chapel Hill NC USA"},{"name":"Department of Biostatistics University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela A.","family":"Groben","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig C.","family":"Carson","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglin","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eloise","family":"Parrish","sequence":"additional","affiliation":[{"name":"Lineberger Comprehensive Cancer Center University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stergios J.","family":"Moschos","sequence":"additional","affiliation":[{"name":"Lineberger Comprehensive Cancer Center University of North Carolina Chapel Hill NC USA"},{"name":"Department of Medicine University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Berwick","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Epidemiology University of New Mexico Albuquerque NM USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Ollila","sequence":"additional","affiliation":[{"name":"Lineberger Comprehensive Cancer Center University of North Carolina Chapel Hill NC USA"},{"name":"Department of Surgery University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Conway","sequence":"additional","affiliation":[{"name":"Lineberger Comprehensive Cancer Center University of North Carolina Chapel Hill NC USA"},{"name":"Department of Epidemiology University of North Carolina Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.23.4799"},{"key":"e_1_2_7_3_1","unstructured":"Broad Institute TCGA Data Analysis Center(2014a).Clustering of Methylation: consensus NMF. 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