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We found that <jats:styled-content style=\"fixed-case\">FK<\/jats:styled-content>506 treatment increased the melanin contents (especially that of eumelanin) in both melanocytes and melanoma cells. This treatment did not affect the transcription levels of tyrosinase, suggesting that <jats:styled-content style=\"fixed-case\">FK<\/jats:styled-content>506 increases melanin synthesis by regulating cellular levels of tyrosinase. Interestingly, <jats:styled-content style=\"fixed-case\">FK<\/jats:styled-content>506 promoted melanosome maturation by increasing melanosomal <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> (a marker of melanosome maturation), thereby enhancing the stability of melanosome\u2010localized tyrosinase. In addition, <jats:styled-content style=\"fixed-case\">FK<\/jats:styled-content>506 enhanced <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content>\u2010mediated melanosome secretion, the uptake of melanosomes by HaCaT cells, and the transfer of melanosomes to keratinocytes co\u2010cultured with melanocytes. Together, these findings suggest that <jats:styled-content style=\"fixed-case\">FK<\/jats:styled-content>506 contributes to melanin synthesis by regulating the maturation of melanosomes and their transfer to keratinocytes. This offers a novel regulatory mechanism through which <jats:styled-content style=\"fixed-case\">FK<\/jats:styled-content>506 and <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> can have a combined effect on melanogenesis.<\/jats:p>","DOI":"10.1111\/pcmr.12443","type":"journal-article","created":{"date-parts":[[2015,11,19]],"date-time":"2015-11-19T04:41:05Z","timestamp":1447908065000},"page":"199-209","source":"Crossref","is-referenced-by-count":32,"title":["<scp>FK<\/scp>506 regulates pigmentation by maturing the melanosome and facilitating their transfer to keratinocytes"],"prefix":"10.1111","volume":"29","author":[{"given":"Hyejung","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Life Sciences The Research Center for Cellular Homeostasis Ewha Womans University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heesung","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Life Sciences 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