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Loss of one of the two subunits<jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content>or strumpellin in mice is lethal, but little is known about their role in mammals in\u00a0vivo. We explored the role of strumpellin, which has previously been linked to hereditary spastic paraplegia, in the mouse melanocytic lineage. Strumpellin knockout in melanocytes revealed abnormal endocytic vesicle morphology but no impairment of migration in\u00a0vitro or in\u00a0vivo and no change in coat colour. Unexpectedly,<jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content>and filamentous actin could still localize to vesicles in the absence of strumpellin, although the shape and size of vesicles was altered. Blue native<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content>revealed the presence of two distinct<jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content>complexes, even in strumpellin knockout cells, revealing that the<jats:styled-content style=\"fixed-case\">WASH<\/jats:styled-content>complex can assemble and localize to endocytic compartments in cells in the absence of strumpellin.<\/jats:p>","DOI":"10.1111\/pcmr.12506","type":"journal-article","created":{"date-parts":[[2016,7,7]],"date-time":"2016-07-07T17:45:37Z","timestamp":1467913537000},"page":"559-571","source":"Crossref","is-referenced-by-count":21,"title":["Loss of strumpellin in the melanocytic lineage impairs the<scp>WASH<\/scp>Complex but does not affect coat colour"],"prefix":"10.1111","volume":"29","author":[{"given":"Benjamin J.","family":"Tyrrell","sequence":"first","affiliation":[{"name":"Cancer Research UK Beatson Institute College of Medical Veterinary and Life Sciences University of 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