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We analyzed the gene expression of young and senescent human melanocytes in culture and compared the gene expression data with a dataset from nevi and melanomas. A concordant altered gene expression was identified in 84 genes when comparing the growth\u2010arrested samples with proliferating samples. <jats:styled-content style=\"fixed-case\">TUBB<\/jats:styled-content>3, which encodes the microtubule protein tubulin <jats:italic>\u03b2<\/jats:italic>\u20103, showed a decreased expression in senescent melanocytes and nevi and was selected for further studies. Depletion of tubulin <jats:italic>\u03b2<\/jats:italic>\u20103 caused accumulation of cells in the G2\/M phase and decreased proliferation and migration. Immunohistochemical assessment of tubulin <jats:italic>\u03b2<\/jats:italic>\u20103 in benign lesions revealed strong staining in the superficial part of the intradermal components, which faded with depth. In contrast, primary melanomas exhibited staining without gradient in a disordered pattern and strong staining of the invasive front. Our results describe an approach to find clinically useful diagnostic biomarkers to more precisely identify cutaneous malignant melanoma and present tubulin <jats:italic>\u03b2<\/jats:italic>\u20103 as a candidate marker.<\/jats:p>","DOI":"10.1111\/pcmr.12572","type":"journal-article","created":{"date-parts":[[2016,12,26]],"date-time":"2016-12-26T14:52:58Z","timestamp":1482763978000},"page":"243-254","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of tubulin <i>\u03b2<\/i>\u20103 as a novel senescence\u2010associated gene in melanocytic malignant transformation"],"prefix":"10.1111","volume":"30","author":[{"given":"Kyriakos","family":"Orfanidis","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology Link\u00f6ping University  Link\u00f6ping Sweden"},{"name":"Department of Clinical and Experimental Medicine Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"W\u00e4ster","sequence":"additional","affiliation":[{"name":"Experimental Pathology Department of Clinical and Experimental Medicine Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Lundmark","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Link\u00f6ping University  Link\u00f6ping Sweden"},{"name":"Department of Clinical Pathology and Clinical Genetics Link\u00f6ping University Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inger","family":"Rosdahl","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology Link\u00f6ping University  Link\u00f6ping Sweden"},{"name":"Department of Clinical and Experimental Medicine Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4075-159X","authenticated-orcid":false,"given":"Karin","family":"\u00d6llinger","sequence":"additional","affiliation":[{"name":"Experimental Pathology Department of Clinical and Experimental Medicine Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02118"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.406"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12738"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1207\/S15327914nc392_21"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2010.10.030"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0531-5565(01)00098-5"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-012-2024-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12594"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/19.2.185"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-030212-183653"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2233"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3470543"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210950"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2014.11.014"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9363"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(96)90157-4"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12363"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/515S110a"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2803"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1971610"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.641"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-11-0899"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2007.00970.x"},{"key":"e_1_2_7_27_1","first-page":"1117","article-title":"The natural history of the pigmented nevus; factors of age and anatomic location","volume":"25","author":"Lund H.Z.","year":"1949","journal-title":"Am. 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