{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T12:19:38Z","timestamp":1765369178646,"version":"3.37.3"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,7,4]],"date-time":"2017-07-04T00:00:00Z","timestamp":1499126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The current methods for melanocyte delivery to depigmented skin are invasive and often require sophisticated approaches. Here, we describe a promising simple and minimally invasive technique based on the dermarolling system. The technique involves preparation of a keratinocyte\/melanocyte suspension prepared by trypsinization from a non\u2010lesioned part of a patient's scalp skin and transepidermal delivery using a dermaroller equipped with 0.2\u2010mm needles. Dermarolling leads to epidermal microinjuries without, however, causing pain or inflammation. The technique was applied to facial depigmented areas of five patients with stable vitiligo that were resistant to conventional UV\/tacrolimus treatment. The efficacy of cell delivery was analysed histologically on punch biopsies, and repigmentation was evaluated photographically and by clinical assessment. We found that the transplanted keratinocytes rapidly degenerated, leading to keratinization, but melanocytes survived and integrated appropriately into the basal layer of the epidermis, leading to excellent repigmentation after 6\u00a0months in three cases and mild repigmentation in two cases. Although based on a small number of patients, we anticipate this technique to become a valid addition to the arsenal of therapeutic approaches to repigmentation in depigmenting disorders.<\/jats:p>","DOI":"10.1111\/pcmr.12600","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T15:06:39Z","timestamp":1495551999000},"page":"493-497","source":"Crossref","is-referenced-by-count":11,"title":["The first transepidermal transplantation of non\u2010cultured epidermal suspension using a dermarolling system in vitiligo: A\u00a0sequential histological and clinical study"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1885-6563","authenticated-orcid":false,"given":"Laila","family":"Benzekri","sequence":"first","affiliation":[{"name":"Pigmentary Disorders Outpatient Clinic Department of Dermatology Mohammed V University in Rabat  Rabat Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvon","family":"Gauthier","sequence":"additional","affiliation":[{"name":"Vitiligo Melasma Research Association  Lacanau France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,4]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"548","article-title":"Sequential immunohistochemical study of depigmenting and repigmenting minigrafts in vitiligo","volume":"13","author":"Abdallah M.","year":"2003","journal-title":"European Journal of Dermatology"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2015.2234"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000334165"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-2077.58529"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/0378-6323.90947"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0190-9622(92)70024-A"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2009.01423.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402270311"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-010-1034-7"},{"issue":"4","key":"e_1_2_5_11_1","first-page":"173","article-title":"A regionwise comparative study of the extent of post punch graft surgical repigmentation in cutaneous achromia","volume":"64","author":"Lahiri K.","year":"1998","journal-title":"Indian Journal of Dermatology"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-1112-4"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1995.tb08715.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12514597"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12492"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2004.02403.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040377"}],"container-title":["Pigment Cell &amp; Melanoma Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpcmr.12600","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pcmr.12600","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:26:42Z","timestamp":1695493602000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pcmr.12600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,4]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/pcmr.12600"],"URL":"https:\/\/doi.org\/10.1111\/pcmr.12600","archive":["Portico"],"relation":{},"ISSN":["1755-1471","1755-148X"],"issn-type":[{"type":"print","value":"1755-1471"},{"type":"electronic","value":"1755-148X"}],"subject":[],"published":{"date-parts":[[2017,7,4]]}}}