{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:14:52Z","timestamp":1790194492809,"version":"4.1.0"},"reference-count":110,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,1,7]],"date-time":"2019-01-07T00:00:00Z","timestamp":1546819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cutaneous melanoma is a skin cancer with increasing incidence. Identification of novel clinical biomarkers able to detect the stage of disease and suggest prognosis could improve treatment and outcome for melanoma patients. Cell\u2010free micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (cf\u2010mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) are the circulating copies of short non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s involved in gene expression regulation. They are released into the interstitial fluid, are detectable in blood and other body fluids and have interesting features of ideal biomarker candidates. They are stable outside the cell, tissue specific, vary along with cancer development and are sensitive to change in the disease course such as progression or therapeutic response. Moreover, they are accessible by non\u2010invasive methods or venipuncture. Some articles have reported different cf\u2010mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s with the potential of diagnostic tools for melanoma staging, recurrence and survival prediction. Although some concordance of results is already emerging, differences in analytical methods, normalization strategies and tumour staging still will require further research and standardization prior to clinical usage of cf\u2010mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> analysis. This article reviews this literature with the aim of contributing to a shared focusing on these new promising tools for melanoma treatment and care.<\/jats:p>","DOI":"10.1111\/pcmr.12755","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T19:06:46Z","timestamp":1543345606000},"page":"486-499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Circulating cell\u2010free micro<scp>RNA<\/scp>s in cutaneous melanoma staging and recurrence or survival prognosis"],"prefix":"10.1111","volume":"32","author":[{"given":"Beatrice","family":"Polini","sequence":"first","affiliation":[{"name":"Department of Pharmacy University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3291-9009","authenticated-orcid":false,"given":"Sara","family":"Carpi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Romanini","sequence":"additional","affiliation":[{"name":"Medical Oncology Unit University Hospital of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Breschi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Nieri","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano","family":"Podest\u00e0","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2017.5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.02.020"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2012.09.015"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00131.2010"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1997"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1755"},{"key":"e_1_2_8_8_1","unstructured":"Carpi S. Polini B. Fogli S. Nieri P. &Romanini A.(2016).Circulating microRNAs in cutaneous melanoma diagnosis and prognosis. Management of malignant melanoma SMGroups 1\u201312."},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.10.11.15777"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.091061"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.07.010"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000452540"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.227611"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914143107"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.6.1891"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2016.1164693"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2016.0486"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-2538"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2015.357"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056824"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","first-page":"14545","DOI":"10.18632\/oncotarget.3859","article-title":"Absolute quantification of cell\u2010free microRNAs in cancer patients","volume":"16","author":"Ferracin M.","year":"2015","journal-title":"Oncotarget"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/1010428317701646"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2014-15-4-r57"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-10-155"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2016.05.005"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.05.027"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.25009"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12200"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2504"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.16.6541"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2015.12.041"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3838"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2012.119"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2010-0103"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201100209"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/JID.2015.355"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2011.11.001"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3965"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2010.12.010"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.12.023"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808830105"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2016.87"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024145"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075184"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2843"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1097\/CAD.0b013e32834dc279"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.suronc.2016.05.017"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90529-Y"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12018"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-262"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2011.00857.x"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.891327"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10731"},{"issue":"3","key":"e_1_2_8_54_1","first-page":"4297","article-title":"Down\u2010regulation of tissue microRNA\u2010126 was associated with poor prognosis in patients with cutaneous melanoma","volume":"8","author":"Lin N.","year":"2015","journal-title":"International Journal of Clinical and Experimental Medicine"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1030"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2015.08.010"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2964"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.07.009"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S128819"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2014.02.001"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22542"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3661"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddl046"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00762-15"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-6-r64"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12346"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2015.10.009"},{"key":"e_1_2_8_68_1","unstructured":"NCCN Melanoma.NCCN Melanoma Version 1.2017 \u2013 November 10 2016."},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-0405"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm4101890"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.939908"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/sigtrans.2015.4"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apradiso.2014.11.014"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-017-0285-9"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043569"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.90"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3198"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-11-0370"},{"key":"e_1_2_8_79_1","unstructured":"miRBase. (2018).miRBase miRNA homo sapiens. Retrieved fromhttp:\/\/www.mirbase.org\/cgi-bin\/mirna_summary.pl?org=hsa"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.477"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1514-5"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24960"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2014.5"},{"key":"e_1_2_8_84_1","unstructured":"SEER Cancer Statistics Factsheets(2007).SEER Cancer Statistics Factsheets: Melanoma of the Skin.Bethesda MD:National Cancer Institute. Retrieved fromhttp:\/\/seer.cancer.gov\/statfacts\/html\/melan.html"},{"key":"e_1_2_8_85_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808263106"},{"key":"e_1_2_8_86_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0b013e328363e485"},{"key":"e_1_2_8_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1800"},{"key":"e_1_2_8_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2015.05.011"},{"key":"e_1_2_8_89_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10109"},{"key":"e_1_2_8_90_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2012-0360"},{"key":"e_1_2_8_91_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12989"},{"key":"e_1_2_8_92_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2014.176"},{"key":"e_1_2_8_93_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2016.4321"},{"key":"e_1_2_8_94_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22332"},{"key":"e_1_2_8_95_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"e_1_2_8_96_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2018.01.001"},{"key":"e_1_2_8_97_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22402"},{"key":"e_1_2_8_98_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2013.01.008"},{"key":"e_1_2_8_99_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0000000000000374"},{"issue":"7","key":"e_1_2_8_100_1","first-page":"673","article-title":"Molecular networks and mechanisms of epithelial\u2010mesenchymal transition regulated by miRNAs in the malignant melanoma cell line","volume":"37","author":"Wang D.","year":"2015","journal-title":"Yi Chuan"},{"issue":"6","key":"e_1_2_8_101_1","first-page":"13","article-title":"Diagnostic and prognostic biomarkers in melanoma","volume":"7","author":"Weinstein D.","year":"2014","journal-title":"Journal of Clinical and Aesthetic Dermatology"},{"key":"e_1_2_8_102_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2010.07.002"},{"key":"e_1_2_8_103_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-017-1784-6"},{"key":"e_1_2_8_104_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.568"},{"key":"e_1_2_8_105_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S107235"},{"key":"e_1_2_8_106_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.5202"},{"key":"e_1_2_8_107_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1079"},{"key":"e_1_2_8_108_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.05.133"},{"key":"e_1_2_8_109_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22032"},{"key":"e_1_2_8_110_1","doi-asserted-by":"publisher","DOI":"10.5732\/cjc.012.10271"},{"key":"e_1_2_8_111_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2015.1030545"}],"container-title":["Pigment Cell &amp; Melanoma Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpcmr.12755","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pcmr.12755","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pcmr.12755","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pcmr.12755","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T01:43:52Z","timestamp":1694223832000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pcmr.12755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,7]]},"references-count":110,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1111\/pcmr.12755"],"URL":"https:\/\/doi.org\/10.1111\/pcmr.12755","archive":["Portico"],"relation":{},"ISSN":["1755-1471","1755-148X"],"issn-type":[{"value":"1755-1471","type":"print"},{"value":"1755-148X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,7]]},"assertion":[{"value":"2018-03-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}