{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:34:51Z","timestamp":1790080491407,"version":"4.0.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T00:00:00Z","timestamp":1578873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2017\/10547\u20108"],"award-info":[{"award-number":["#2017\/10547\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2017\/20745\u20101"],"award-info":[{"award-number":["#2017\/20745\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acral lentiginous melanoma (ALM) is a rare subtype of melanoma with aggressive behavior. IMPDH enzyme, involved in de novo GTP biosynthesis, has been reported to assemble into large filamentary structures called rods\/rings (RR) or cytoophidium (cellular snakes). RR assembly induces a hyperactive state in IMPDH, usually to supply a high demand for GTP nucleotides, such as in highly proliferative cells. We investigate whether aggressive melanoma tumor cells present IMPDH\u2010based RR structures. Forty\u2010five ALM paraffin\u2010embedded tissue samples and 59 melanocytic nevi were probed with anti\u2010IMPDH2 antibody. Both the rod\u2010 and ring\u2010shaped RR could be observed, with higher frequency in ALM. ROC curve analyzing the proportions of RR\u2010positive cells in ALM versus nevi yielded a 0.88 AUC. Using the cutoff of 5.5% RR\u2010positive cells, there was a sensitivity of 80% and specificity of 85% for ALM diagnosis. In ALM, 36 (80%) showed RR frequency above the cutoff, being classified as RR\u2010positive, compared with only 9 (15%) of the nevi (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001). Histopathology showed that 71% of the RR\u2010positive specimens presented Breslow thickness\u00a0&gt;\u00a04.0mm, compared with only 29% in the RR\u2010low\/negative (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.039). We propose that screening for RR structures in biopsy specimens may be a valuable tool helping differentiate ALM from nevi and accessing tumor malignancy.<\/jats:p>","DOI":"10.1111\/pcmr.12854","type":"journal-article","created":{"date-parts":[[2019,12,28]],"date-time":"2019-12-28T07:55:02Z","timestamp":1577519702000},"page":"490-497","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["IMP dehydrogenase rod\/ring structures in acral melanomas"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0660-2857","authenticated-orcid":false,"given":"Gerson D.","family":"Keppeke","sequence":"first","affiliation":[{"name":"Rheumatology Division Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4010-4109","authenticated-orcid":false,"given":"Denise","family":"Barcelos","sequence":"additional","affiliation":[{"name":"Department of Pathology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6208-0179","authenticated-orcid":false,"given":"Mariana","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Pathology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7492-4319","authenticated-orcid":false,"given":"Andr\u00e9ia N.","family":"Comodo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiane P.","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Department of Pathology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9673-4030","authenticated-orcid":false,"given":"Leonardo","family":"Cardili","sequence":"additional","affiliation":[{"name":"Department of Pathology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2943-8244","authenticated-orcid":false,"given":"Fernando C. 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