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However, some patients do not respond, and variable immune\u2010related adverse events have been reported. Therefore, more effective and antigen\u2010specific immune therapies are urgently needed. We previously reported the efficacy of an immune cell therapy with immortalized myeloid cells derived from induced pluripotent stem cells (iPS\u2010ML). In this study, we generated OX40L\u2010overexpressing iPS\u2010ML (iPS\u2010ML\u2010Zsgreen\u2010OX40L) and investigated their characteristics and in vivo efficacy against mouse melanoma. We found that iPS\u2010ML\u2010Zsgreen\u2010OX40L suppressed the progression of B16\u2010BL6 melanoma, and prolonged survival of mice with ovalbumin (OVA)\u2010expressing B16 melanoma (MO4). The number of antigen\u2010specific CD8<jats:sup>+<\/jats:sup> T cells was higher in spleen cells treated with OVA peptide\u2010pulsed iPS\u2010ML\u2010Zsgreen\u2010OX40L than in those without OX40L. The OVA peptide\u2010pulsed iPS\u2010ML\u2010Zsgreen\u2010OX40L significantly increased the number of tumor\u2010infiltrating T lymphocytes (TILs) in MO4 tumor. Flow cytometry showed decreased regulatory T cells but increased effector and effector memory T cells among the TILs. Although we plan to use allogeneic iPS\u2010ML in the clinical applications, iPS\u2010ML showed the tumorgenicity in the syngeneic mice model. Incorporating the suicide gene is necessary to ensure the safety in the future study. Collectively, these results indicate that iPS\u2010ML\u2010Zsgreen\u2010OX40L therapy might be a new method for antigen\u2010specific cancer immunotherapy.<\/jats:p>","DOI":"10.1111\/pcmr.12887","type":"journal-article","created":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T21:35:49Z","timestamp":1588282549000},"page":"744-755","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Induced pluripotent stem cell\u2010derived myeloid cells expressing OX40 ligand amplify antigen\u2010specific T cells in advanced melanoma"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1709-6796","authenticated-orcid":false,"given":"Toshihiro","family":"Kimura","sequence":"first","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University  Kumamoto 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