{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T22:19:38Z","timestamp":1775254778110,"version":"3.50.1"},"reference-count":69,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T00:00:00Z","timestamp":1671408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012069","name":"Hoyu Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conditional and inducible gene targeting using Cre\/<jats:italic>loxP<\/jats:italic>\u2010mediated recombination is a powerful reverse genetics approach used to study spatiotemporal gene functions in specified cell types. To enable temporal gene manipulation in the melanocyte lineage, we established a novel inducible Cre\u2010driver mouse line by targeting an all\u2010in\u2010one tetracycline\/doxycycline (Dox)\u2010inducible Cre expression cassette into the <jats:italic>Pmel<\/jats:italic> locus (<jats:italic>Pmel<\/jats:italic><jats:sup><jats:italic>P2A\u2010TetON3G\u2010TRE3G\u2010iCre<\/jats:italic><\/jats:sup>), a gene locus preferentially expressed in pigment cells. By crossing these Cre\u2010driver mice with a strong Cre\u2010reporter mouse line, <jats:italic>Gt(ROSA)26Sor<\/jats:italic><jats:sup><jats:italic>tm9(CAG\u2010tdTomato)Hze<\/jats:italic><\/jats:sup>, we show the effectiveness of the <jats:italic>Pmel<\/jats:italic><jats:sup><jats:italic>P2A\u2010TetON3G\u2010TRE3G\u2010iCre<\/jats:italic><\/jats:sup> mouse line in facilitating Dox\u2010inducible Cre\/<jats:italic>loxP<\/jats:italic> recombination in a wide variety of pigment cell lineages including hair follicle melanocytes and their stem cells. Furthermore, to demonstrate proof of concept, we ablated Notch signaling postnatally in the <jats:italic>Pmel<\/jats:italic><jats:sup><jats:italic>P2A\u2010TetON3G\u2010TRE3G\u2010iCre<\/jats:italic><\/jats:sup> mice. In agreement with the previously reported phenotype, induced ablation of Notch signaling in the melanocyte lineage resulted in premature hair graying, demonstrating the utility of the <jats:italic>Pmel<\/jats:italic><jats:sup><jats:italic>P2A\u2010TetON3G\u2010TRE3G\u2010iCre<\/jats:italic><\/jats:sup> allele. Therefore, the <jats:italic>Pmel<\/jats:italic><jats:sup><jats:italic>P2A\u2010TetON3G\u2010TRE3G\u2010iCre<\/jats:italic><\/jats:sup> mouse line is suitable for assessing gene functions in melanocytes using an in vivo inducible reverse genetics approach. Furthermore, we unexpectedly identified previously unrecognized PMEL\u2010expressing cells in non\u2010pigmentary organs in the mice, suggesting unanticipated functions of PMEL other than melanosome formation.<\/jats:p>","DOI":"10.1111\/pcmr.13074","type":"journal-article","created":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T15:59:00Z","timestamp":1667318340000},"page":"53-70","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Generation of <i>Pmel<\/i>\u2010dependent conditional and inducible Cre\u2010driver mouse line for melanocytic\u2010targeted gene manipulation"],"prefix":"10.1111","volume":"36","author":[{"given":"Morsheda","family":"Nasrin","sequence":"first","affiliation":[{"name":"Department of Regeneration and Applied Biomedical Sciences, Graduate School of Medicine Gifu University  Gifu 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