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Its expression has prognostic and predictive value in CLL and is prognostic in breast cancer. Its significance in melanoma is unclear. We analyzed <jats:italic>CD52<\/jats:italic> mRNA expression data from tumor bulk tissues of <jats:italic>N<\/jats:italic>\u2009=\u2009445 untreated melanoma patients from The Cancer Genome Atlas (TCGA) Research Network and of <jats:italic>N<\/jats:italic>\u2009=\u2009121 melanoma patients undergoing anti\u2010PD\u20101 immune checkpoint blockade (ICB) with regard to outcome (overall survival [OS], disease control [DC], and progression\u2010free survival [PFS]), single\u2010cell RNA\u2010Seq data of <jats:italic>N<\/jats:italic>\u2009=\u20094645 cells from <jats:italic>N<\/jats:italic>\u2009=\u200919 melanoma tissues, and <jats:italic>N<\/jats:italic>\u2009=\u200915,457 cells from normal skin provided by <jats:italic>N<\/jats:italic>\u2009=\u20095 donors. Higher <jats:italic>CD52<\/jats:italic> mRNA expression was associated with favorable OS (hazard ratio (HR)\u2009=\u20090.820, [95% CI 0.734\u20130.916], <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) in non\u2010ICB\u2010treated melanoma and with PFS (HR\u2009=\u20090.875, [95% CI 0.775\u20130.989], <jats:italic>p<\/jats:italic>\u2009=\u2009.033) and DC (<jats:italic>p<\/jats:italic>\u2009=\u2009.005) in ICB\u2010treated melanoma. <jats:italic>CD52<\/jats:italic> expression correlated significantly with distinct immune cell subsets and correlated negatively with immune checkpoint expression in T cells. Moreover, our results suggest <jats:italic>CD52<\/jats:italic> expression by a certain type of tissue\u2010resident macrophages. <jats:italic>CD52<\/jats:italic> mRNA was expressed in a small subgroup (8%) of immune checkpoint coexpressing melanoma cells. <jats:italic>CD52<\/jats:italic> expression is associated with features of ICB response in melanoma. Concomitant ICB and anti\u2010CD52 treatment requires critical review.<\/jats:p>","DOI":"10.1111\/pcmr.13151","type":"journal-article","created":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T03:44:45Z","timestamp":1700279085000},"page":"309-315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp><i>CD52<\/i> mRNA<\/scp> expression predicts prognosis and response to immune checkpoint blockade in melanoma"],"prefix":"10.1111","volume":"37","author":[{"given":"Luka","family":"de Vos\u2010Hillebrand","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergology University Medical Center Bonn (UKB)  Bonn Germany"},{"name":"Department of Otorhinolaryngology University Medical Center Bonn (UKB)  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Fietz","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology 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