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Here, we examined the effects of 6\u2010week topical antibiotic treatment on vitiligo\u2010prone pmel\u20101 mice. Bacitracin, Neosporin, or Vaseline were applied to one denuded flank, while the contralateral flank was treated with Vaseline in all mice. Ventral depigmentation was quantified weekly. We found that topical Neosporin treatment significantly reduced depigmentation and exhibited effects beyond the treated area, while Bacitracin ointment had no effect. Stool samples collected from four representative mice\/group during treatment revealed that Neosporin treatment aligned with reduced abundance of the <jats:italic>Alistipes<\/jats:italic> genus in the gut, while relevant changes to the skin microbiome at end point were less apparent. Either antibiotic treatment led to reduced expression of MR1, potentially limiting mucosal\u2010associated invariant T\u2010cell activation, while Neosporin\u2010treated skin selectively revealed significantly reduced CD8+ T\u2010cell abundance. The latter finding aligned with reduced expression of multiple inflammatory markers and markedly increased regulatory T\u2010cell density. Our studies on favorable skin and oral antibiotic treatment share the neomycin compound, and in either case, microbial changes were most apparent in stool samples. Taken together, neomycin\u2010containing antibiotic applications can mediate skin Treg infiltration to limit vitiligo development. Our study highlights the therapeutic potential of short\u2010term antibiotic applications to limit depigmentation vitiligo.<\/jats:p>","DOI":"10.1111\/pcmr.13164","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T05:51:12Z","timestamp":1709617872000},"page":"583-596","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Topical antibiotics limit depigmentation in a mouse model of\u00a0vitiligo"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0225-0874","authenticated-orcid":false,"given":"Ahmed Ahmed","family":"Touni","sequence":"first","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine Minia University  Minia Egypt"},{"name":"Department of Dermatology, Feinberg School of Medicine Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Sohn","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Feinberg School of Medicine Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cormac","family":"Cosgrove","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Feinberg School of Medicine Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5777-0116","authenticated-orcid":false,"given":"Rohan S.","family":"Shivde","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Feinberg School of Medicine Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9031-2240","authenticated-orcid":false,"given":"Emilia R.","family":"Dellacecca","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Feinberg School of Medicine Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha T. A.","family":"Abdel\u2010Aziz","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kettil","family":"Cedercreutz","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Feinberg School of Medicine Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan J.","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Genomics and Microbiome Core Facility Rush University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossam","family":"Abdel\u2010Wahab","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6853-4007","authenticated-orcid":false,"given":"I. Caroline","family":"Le\u00a0Poole","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Feinberg School of Medicine Northwestern University  Chicago Illinois USA"},{"name":"Department of Microbiology and Immunology, Robert H. Lurie Comprehensive Cancer Center Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-2618-1-31"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2010.11.061"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.21.6.3671"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2022.03.019"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2021.01.036"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.540"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(23)00457\u20109"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/dth.15180"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2019.08.435"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharep.2013.09.007"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2015.08.007"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep18761"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20020283"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-019-0444-8"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan4236"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02759"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115835"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12933"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2019.1665973"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3499"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2010.00688.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.131029.111"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12957"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/0963689720913876"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2021.04.003"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-017-1446-2"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.4103\/injr.injr_210_20"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aao3290"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-21-0808"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.15885"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3005127"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.581433"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-021-00391-w"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.592248"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030590"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.790125"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2016.11.014"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24441"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpim.63"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2019.06.013"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-13-13"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40257-017-0298-5"},{"issue":"5","key":"e_1_2_7_44_1","first-page":"1037","article-title":"Gut\u2010skin Axis: Unravelling the connection between the gut microbiome and psoriasis","volume":"10","author":"Thye A. 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