{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:27:57Z","timestamp":1789540077686,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:00:00Z","timestamp":1739059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["259332240\/RTG 2099"],"award-info":[{"award-number":["259332240\/RTG 2099"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Cancer\u2010associated fibroblasts (CAFs) represent a central cell population of the tumor microenvironment (TME). Recently, single\u2010cell RNA\u2010sequencing (scRNA\u2010seq) analyses of primary tumors of different cancer entities yielded different classifications of CAF subsets underscoring the heterogeneity of CAFs within the TME. Here, we analyzed the transcriptional signatures of approximately 8400 CAFs and normal fibroblasts by scRNA\u2010seq and compared genetic profiles of CAFs from murine melanoma primary tumors to CAFs from corresponding melanoma lung metastases. This revealed distinct subsets for primary tumor and metastasis\u2010specific CAF populations, respectively. Combined with the spatial characterization of metastasis CAFs at the RNA and protein level, scRNA analyses indicate tumor\u2010dependent crosstalk between neutrophils and CAFs, mediated via SAA3 and IL1b\u2010related signaling pathways, which can be recapitulated in\u00a0vitro. Analyzing tissue sections of human patient samples, this interaction was found to be present in human melanoma metastasis. Taken together, our data highlight unique characteristics of metastasis CAFs with potential therapeutic impact for melanoma metastasis.<\/jats:p>","DOI":"10.1111\/pcmr.70002","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T06:41:20Z","timestamp":1739256080000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A Unique Signature for Cancer\u2010Associated Fibroblasts in Melanoma Metastases"],"prefix":"10.1111","volume":"38","author":[{"given":"Saskia","family":"Tauch","sequence":"first","affiliation":[{"name":"Division Signal Transduction and Growth Control German Cancer Research Center (DKFZ\u2010ZMBH Alliance)  Heidelberg Germany"},{"name":"Faculty of Biosciences Heidelberg University  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joschka","family":"Hey","sequence":"additional","affiliation":[{"name":"Division of Cancer Epigenomics German 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