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This study investigates the hypothesis that UV radiation induces chemiexcitation in skin through the formation of triplet excited carbonyls, which may transfer energy to melanin and contribute to oxidative stress even after the termination\u00a0of UV exposure. Using porcine skin as a model, we demonstrate that UV exposure leads to lipid peroxidation and the subsequent formation of organic radicals, including carbon\u2010centered (alkyl) and oxygen\u2010centered (peroxyl and alkoxyl) species, as detected by EPR spin\u2010trapping spectroscopy. HPLC\u2010MS analysis revealed that short\u2010chain carbonyl compounds, such as formaldehyde and acetaldehyde, are the predominant electronically excited species in direct chemiexcitation. These triplet carbonyls can transfer their excitation energy to melanin through photon emission (radiative transfer) or direct electron exchange (non\u2010radiative transfer), forming melanin\u2010based secondary excited states via indirect chemiexcitation. These findings highlight a novel, light\u2010independent mechanism of post\u2010UV exposure oxidative damage in the skin and suggest a possible role for chemiexcitation in processes such as photoaging and photocarcinogenesis.<\/jats:p>","DOI":"10.1111\/pcmr.70060","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T04:16:23Z","timestamp":1760501783000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Chemiexcitation in Ex\u00a0Vivo Porcine Skin Model"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9126-2011","authenticated-orcid":false,"given":"Pavel","family":"Posp\u00ed\u0161il","sequence":"first","affiliation":[{"name":"Department of Biophysics Faculty of Science, Palack\u00fd University  Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5484-5381","authenticated-orcid":false,"given":"Vendula","family":"Paculov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biophysics Faculty of Science, Palack\u00fd University  Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2009-8987","authenticated-orcid":false,"given":"Ankush","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Biophysics Faculty of Science, Palack\u00fd University  Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9294-3271","authenticated-orcid":false,"given":"Michal","family":"Berecka","sequence":"additional","affiliation":[{"name":"Department of Biophysics Faculty of Science, Palack\u00fd University  Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Chemical and Biological Generation of Excited States","author":"Adam W.","year":"1982"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr0300035"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2009.08.003"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CP08154A"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.12716"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.13470"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00787"},{"key":"e_1_2_10_9_1","first-page":"17","volume-title":"Hydrogen Peroxide Metabolism in Health and Disease","author":"Bechara E.","year":"2017"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0185-9"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700340"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2013.12.016"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.13781"},{"issue":"2","key":"e_1_2_10_14_1","first-page":"136","article-title":"Sunlight and Sunburn in Human Skin Cancer: p53, Apoptosis, and Tumor Promotion","volume":"1","author":"Brash D.","year":"1996","journal-title":"Journal of Investigative Dermatology. 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