{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T10:07:34Z","timestamp":1764151654827,"version":"3.46.0"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100016966","name":"Bethune Charitable Foundation","doi-asserted-by":"publisher","award":["J202301E036"],"award-info":[{"award-number":["J202301E036"]}],"id":[{"id":"10.13039\/100016966","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Dyschromatosis universalis hereditaria (DUH) is characterized by autosomal dominant inheritance and widespread involvement of hyperpigmentation and depigmentation. In our previous study, we identified mutations in the SH3 domain\u2010containing protein 1 (\n                    <jats:italic>SASH1<\/jats:italic>\n                    ) associated with the DUH phenotype in Chinese families and predicted that the\n                    <jats:italic>SASH1<\/jats:italic>\n                    \/\n                    <jats:italic>THBS1<\/jats:italic>\n                    \/TGF\u2010\u03b21 signaling pathway mediates melanin production and melanocyte transport. However, the molecular regulatory mechanisms remain unclear. By modulating the expression of\n                    <jats:italic>SASH1<\/jats:italic>\n                    and\n                    <jats:italic>THBS1<\/jats:italic>\n                    , we assessed the expression of genes within the\n                    <jats:italic>SASH1<\/jats:italic>\n                    \/\n                    <jats:italic>THBS1<\/jats:italic>\n                    \/TGF\u2010\u03b21 pathway and evaluated cell phenotypes and melanin synthesis in the A375 and PIG1 cell lines. Through in\u00a0vivo subcutaneous injection of\n                    <jats:italic>SASH1<\/jats:italic>\n                    knockdown A375 cells into nude mice, we tested whether\n                    <jats:italic>SASH1<\/jats:italic>\n                    \u2010TGF\u2010\u03b21 signaling may regulate cancer growth. Our findings demonstrated that\n                    <jats:italic>SASH1<\/jats:italic>\n                    inhibits proliferation, migration, invasion, epithelial\u2013mesenchymal transition, and promotes melanin synthesis through TGF\u2010\u03b21 signaling, while\n                    <jats:italic>THBS1<\/jats:italic>\n                    counteracts the increase in TGF\u2010\u03b21 levels induced by\n                    <jats:italic>SASH1<\/jats:italic>\n                    knockdown. We further showed that\n                    <jats:italic>SASH1<\/jats:italic>\n                    suppresses TGF\u2010\u03b21 through its regulatory effect on\n                    <jats:italic>THBS1<\/jats:italic>\n                    , thereby inhibiting melanin metastasis and promoting melanin synthesis, which offers potential therapeutic insights into the modulation of TGF\u2010\u03b21.\n                  <\/jats:p>","DOI":"10.1111\/pcmr.70064","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T17:09:20Z","timestamp":1764004160000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>SASH1<\/scp>\n                    Modulates Melanin Synthesis and Melanoma Cell Metastasis via Suppression of the\n                    <scp>TGF<\/scp>\n                    \u2010\u03b2 Signaling Pathway"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5513-3140","authenticated-orcid":false,"given":"Hongzhou","family":"Cui","sequence":"first","affiliation":[{"name":"First Clinical Medical College of Shanxi Medical University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0032-7221","authenticated-orcid":false,"given":"Jianhua","family":"Hao","sequence":"additional","affiliation":[{"name":"First Clinical Medical College of Shanxi Medical University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Jiangsu Province Hospital  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Han","sequence":"additional","affiliation":[{"name":"First Clinical Medical College of Shanxi Medical University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honggang","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The Second People's Hospital of Changzhi  Changzhi Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Chinese PLA General Hospital (CPLAGH)  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0267-8184","authenticated-orcid":false,"given":"Bo","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Dermatology Anhui Medical University  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Ge","sequence":"additional","affiliation":[{"name":"The Physical Examination Center The First Hospital of Shanxi Medical University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxia","family":"He","sequence":"additional","affiliation":[{"name":"First Clinical Medical College of Shanxi Medical University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Ren","sequence":"additional","affiliation":[{"name":"First Clinical Medical College of Shanxi Medical University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuping","family":"Guo","sequence":"additional","affiliation":[{"name":"First Clinical Medical College of Shanxi Medical University  Taiyuan Shanxi 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