{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T19:58:18Z","timestamp":1774641498451,"version":"3.50.1"},"reference-count":79,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    At the 2025 ESPCR (\n                    <jats:italic>European Society for Pigment Cell Research<\/jats:italic>\n                    ) meeting in Erlangen, a workshop on \u201cPigment Cell Models: Sensitivity, Innovation, and the Challenges of Cell Culture\u201d brought together researchers to discuss technical, methodological, and reproducibility issues in culturing melanocytes, keratinocytes, fibroblasts, and melanoma cells. The discussion between experts in the field highlighted key and recurrent pitfalls affecting experimental outcomes, including low\u2010density seeding, temperature fluctuations, over\u2010passaging, and mycoplasma contamination, as well as sources of variability arising from media composition, batch effects, and environmental conditions. Importantly, the workshop distinguished between practices supported by evidence and consensus\u2010based guidance derived from collective expert experience. Species\u2010 and donor\u2010specific differences, especially between human, mouse, and zebrafish melanocyte models, were identified as additional major determinants of experimental variability. Emerging systems, including human and mouse pluripotent stem cell (PSC)\u2010derived melanocytes, as well as avian and zebrafish melanoma lines, were discussed for their complementary mechanistic and translational value. Overall, the workshop concluded that transparent documentation, explicit reporting standards, and shared best practices are essential to improve reproducibility and further advance pigment cell research.\n                  <\/jats:p>","DOI":"10.1111\/pcmr.70084","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T03:25:27Z","timestamp":1773199527000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Short Report on Melanocyte\/Melanoma Culture, Senescence, and Reproducibility"],"prefix":"10.1111","volume":"39","author":[{"given":"Lionel","family":"Larue","sequence":"first","affiliation":[{"name":"INSERM U1021, Normal and Pathological Development of Melanocytes, Institut Curie PSL Research University  Orsay France"},{"name":"CNRS UMR3347 Paris\u2010Sud University, University Paris\u2010Saclay  Orsay France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"ESPCR Working Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8867-2407","authenticated-orcid":false,"given":"Duarte C.","family":"Barral","sequence":"additional","affiliation":[{"name":"iNOVA4Health, NOVA Medical School, Faculdade de Ci\u00eancias M\u00e9dicas, NMS, FCM Universidade NOVA de Lisboa  Lisboa Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronique","family":"Delmas","sequence":"additional","affiliation":[{"name":"INSERM U1021, Normal and Pathological Development of Melanocytes, Institut Curie PSL Research University  Orsay France"},{"name":"CNRS UMR3347 Paris\u2010Sud University, University Paris\u2010Saclay  Orsay France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Egea\u2010Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy, University Hospital LMU Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Aldea","sequence":"additional","affiliation":[{"name":"INSERM, Marseille Medical Genetics, U1251, Institut MarMaRa Aix Marseille University  Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0201-3799","authenticated-orcid":false,"given":"Heather C.","family":"Etchevers","sequence":"additional","affiliation":[{"name":"INSERM, Marseille Medical Genetics, U1251, Institut MarMaRa Aix Marseille University  Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Dominique","family":"Galibert","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Genomics, CNRS, INSERM, IGDR (Institut de G\u00e9n\u00e9tique et D\u00e9veloppement de Rennes)\u2014UMR6290, ERL U1305\u2014Hospital University of Rennes Univ. Rennes  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert N.","family":"Kelsh","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Bath  Bath UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4523-3815","authenticated-orcid":false,"given":"Luisa","family":"Lanfrancone","sequence":"additional","affiliation":[{"name":"Department of Experimental Oncology IEO, European Institute of Oncology IRCCS  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1053-6979","authenticated-orcid":false,"given":"Michele","family":"Madigan","sequence":"additional","affiliation":[{"name":"Optometry and Vision Science UNSW Sydney  Sydney Australia"},{"name":"Save Sight Institute Sydney University  Sydney 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