{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T04:47:25Z","timestamp":1785818845250,"version":"3.56.0"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01\u2010CA249229"],"award-info":[{"award-number":["R01\u2010CA249229"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["T32 CA163177"],"award-info":[{"award-number":["T32 CA163177"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CCSG P30CA044579\u201032"],"award-info":[{"award-number":["CCSG P30CA044579\u201032"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR003015"],"award-info":[{"award-number":["UL1TR003015"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["KL2TR003016"],"award-info":[{"award-number":["KL2TR003016"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In\u2010transit melanoma (ITM) is a unique presentation of metastatic cutaneous melanoma associated with therapeutic challenges. Despite its clinical importance, the molecular drivers of ITM remain poorly defined. We aimed to characterize the mutational landscape of ITM and identify genetic alterations distinguishing it from other melanoma metastases. Tumor samples from 528 patients in the MSK\u2010IMPACT dataset, comprised of over 300 cancer\u2010related genes, were analyzed. Samples were classified as primary, in\u2010transit, regional lymph node, or distant metastases. Driver mutation frequencies were compared across groups, and mutual information (MI) and principal component analysis (PCA) were used to identify ITM\u2010associated genes and mutation patterns. NRAS Q61 mutations were enriched in ITM compared with other melanoma sites, while NF1 mutations were less common. Exploratory MI, PCA, and pairwise analyses identified recurrent NRAS\n                    <jats:sup>MUT<\/jats:sup>\n                    \/wild\u2010type gene patterns that may distinguish ITM from other melanoma samples. Genes retained in the wild\u2010type state alongside NRAS\n                    <jats:sup>MUT<\/jats:sup>\n                    were associated with PI3K\/AKT\/mTOR, TGF\u2010\u03b2, and E2F\u2010related pathways. Collectively, these findings suggest that ITM is enriched for NRAS Q61 mutations and may have a comparatively lower co\u2010mutation burden, providing a basis for future studies of ITM biology and clinical behavior.\n                  <\/jats:p>","DOI":"10.1111\/pcmr.70112","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T04:49:49Z","timestamp":1784090989000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterizing the Mutational Landscape of In\u2010Transit Melanoma Metastases"],"prefix":"10.1111","volume":"39","author":[{"given":"Luisa Quesada","family":"Camacho","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamad","family":"El Moheb","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"},{"name":"UVA Comprehensive Cancer Center, University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9907-8923","authenticated-orcid":false,"given":"Mackenzie M.","family":"Mayhew","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret G.","family":"Mercante","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily G.","family":"Tocco","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1274-1399","authenticated-orcid":false,"given":"Nidhi","family":"Kuchimanchi","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan J.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6962-9802","authenticated-orcid":false,"given":"Kaelyn C.","family":"Cummins","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn T.","family":"Dengel","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"},{"name":"UVA Comprehensive Cancer Center, University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan","family":"Tsung","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"},{"name":"UVA Comprehensive Cancer Center, University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Craig L.","family":"Slingluff","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"},{"name":"UVA Comprehensive Cancer Center, University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Fallahi\u2010Sichani","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Virginia  Charlottesville Virginia USA"},{"name":"UVA Comprehensive Cancer Center, University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell G.","family":"Witt","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Virginia  Charlottesville Virginia USA"},{"name":"UVA Comprehensive Cancer Center, University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0931262100"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2014.12.006"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.34807"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2022.03.041"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.Ccr\u201013\u20103270"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388\u2010022\u201002244\u20107"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201001947\u2010w"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24473"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0002\u20109440(10)64088\u20106"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc\u20102019\u2010000440"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434\u2010019\u201007965\u2010w"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.Can\u201013\u20102625"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-021-10100-3"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.ccr\u201003\u20100086"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1216013110"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2021.102238"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16173065"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.Can\u201024\u20100693"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476\u20104598\u201013\u201045"}],"container-title":["Pigment 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