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A dearth of well\u2010established and characterized melanoma cell lines that reflect rare subtypes impedes research on these lethal diseases. We present a protocol developed by exploring various combinations of established techniques that we used to generate mucosal and acral cell lines from patient tissues and patient derived xenografts.<\/jats:p>","DOI":"10.1111\/pcmr.70117","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T05:32:16Z","timestamp":1788586336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Technique for the Generation of Mucosal and Acral Melanoma Cell Lines"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7013-1132","authenticated-orcid":false,"given":"Ishani","family":"Banik","sequence":"first","affiliation":[{"name":"Department of Dermatology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sugandha","family":"Basu","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9410-6866","authenticated-orcid":false,"given":"Austin J.","family":"Escobar","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2499-6618","authenticated-orcid":false,"given":"Veronica","family":"Steri","sequence":"additional","affiliation":[{"name":"University of California, Pre\u2010Clinical Therapeutics Core  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7494-1127","authenticated-orcid":false,"given":"Juan A.","family":"Camara Serrano","sequence":"additional","affiliation":[{"name":"University of California, Pre\u2010Clinical Therapeutics Core  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Phojanakong","sequence":"additional","affiliation":[{"name":"University of California, Pre\u2010Clinical Therapeutics Core  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8989-7093","authenticated-orcid":false,"given":"Noel Cruz","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7705-1813","authenticated-orcid":false,"given":"Katy K.","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Medicine University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin A.","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology &amp; Reproductive Sciences University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6617-8421","authenticated-orcid":false,"given":"Adil","family":"Daud","sequence":"additional","affiliation":[{"name":"Department of Medicine University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8121-3726","authenticated-orcid":false,"given":"Ivan H.","family":"El\u2010Sayed","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1553-4366","authenticated-orcid":false,"given":"Anastasia","family":"Samarkina","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3233-2371","authenticated-orcid":false,"given":"Joy","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie D.","family":"Aquino","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Xiao","sequence":"additional","affiliation":[{"name":"The Wistar Melanoma Research Center  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenhard","family":"Herlyn","sequence":"additional","affiliation":[{"name":"The Wistar Melanoma Research Center  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3941-3561","authenticated-orcid":false,"given":"Iwei","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Francisco California USA"},{"name":"Department of Pathology University of California  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.3789"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1984.tb04631.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0000000000000764"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.6.2018"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2012.01016.x"},{"key":"e_1_2_11_7_1","first-page":"11","article-title":"An Update on Mucosal Melanoma: Future Directions","volume":"27","author":"Goldemberg D. 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